Abstract:

BACKGROUND: Researches show that the expression of interleukin-1β is elevated in atherosclerosis vascular cells.
OBJECTIVE: To investigate the effect of early growth response-1 small interfering RNA on the expression of interleukin-1β mRNA induced by cigarette smoke extract in rat aortic vascular smooth muscle cells.
METHODS: Rat aortic vascular smooth muscle cells were cultured in vitro, then treated with cigarette smoke extracts of different volume fractions (0%, 5%, 10%, 20% and 40%) to screen for the optimal volume fraction. On that basis, the cells were treated for 0, 8, 16 and 24 hours to determine the optimal intervention time. In the end, the expression of growth response-1 was shut down by growth response-1 interfering RNA.
RESULTS AND CONCLUSION: According to RT-PCR, cigarette smoke extract was able to induce interleukin-1β mRNA expression in a dose-dependent and time-dependent manner in rat aortic vascular smooth muscle cells. The optimal intervention time and volume fraction of cigarette smoke extract were 8 hours and 10%. Results presented that cells transfected with small interfering RNA targeting growth response-1 for 8 hours showed a significant reduction in interleukin-1β mRNA expression (P < 0.05), in response to 10% cigarette smoke extract treatment. It proves that early growth response-1 small interfering RNA can inhibit the expression of interleukin-1β mRNA induced by cigarette smoke in rat aortic vascular smooth muscle cells.