Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (31): 5831-5834.doi: 10.3969/j.issn.1673-8225.2011.31.031

Previous Articles     Next Articles

Expression of spermatogenesis-related genes Dazl, Pgk2, Prm2 and apoptosis-related gene bax in testicle and epididymis tissue from ovarian graft heterosexual recipients

Peng Nan-ni1,2, Xue Li-qun1, Yuan An-wen1, Yan Qing1, Ling Li-jun1, Wu Shan1, Zou Hong-yan2, Wu Fang-gui2   

  1. 1Colleges of Veterinary Medicine and Animal Science & Technology, Hunan Agricultural University, Changsha 410128, Hunan Province, China; 2Reproductive Medical Center of Shenzhen Fifth People’s Hospital, Shenzhen 518000, Guangdong Province, China
  • Received:2010-03-04 Revised:2011-05-04 Online:2011-07-30 Published:2011-07-30
  • Contact: Yuan An-wen, Doctor, Associate professor, Colleges of Veterinary Medicine and Animal Science & Technology, Hunan Agricultural University, Changsha 410128, Hunan Province, China yuananwen@yahoo. com.cn
  • About author:Peng Nan-ni☆, Doctor, Associate chief physician, Colleges of Veterinary Medicine and Animal Science & Technology, Hunan Agricultural University, Changsha 410128, Hunan Province, China; Reproductive Medical Center of Shenzhen Fifth People’s Hospital, Shenzhen 518000, Guangdong Province, China nnpeng@126.com
  • Supported by:

    Key Science and Technology Program of Hunan Province, No.01NKY1002-01*; Science and Technology Program of Human Province, No. 2007-TP4024*;  a grant from Science and Technology Bureau of Luohu District of Shenzhen City, No.200806*

PDF

502

Abstract:

BACKGROUND: Dazl, Pgk2, Prm2 and apoptosis-related gene bax are involved in the regulation of spermatogenesis. The abnormal expression and deletions of these genes often affect spermatogenesis, and even lead to infertility.
OBJECTIVE: To investigate the effects of ovarian transplantation on the expression levels of spermatogenesis-related genes in heterosexual recipients.
METHODS: Ovaries from 1-day-old mice were transplanted under the kidney capsule of male recipient mice, and recipients were treated with or without hormone. Ovarian grafts were observed 21 days after transplantation. Tissues including testis and epididymis were collected from mice with good developmental follicles. Total RNAs were extracted and expression levels of genes were determined using semi-quantitative RT-PCR. Normal male mice with the same age were used as controls.
RESULTS AND CONCLUSION: Gene Prm2 expression in the transplanted group treated with gonadotrophine was significantly higher than that in the control group (P < 0.05), and there was no significant difference in expression level of other genes between all groups (P  > 0.05). These data confirmed that ovarian grafts have no effects on the expression levels of spermatogenesis-and apoptosis-related genes in the mouse model with male and female gonads. This may suggest that ovarian transplantation has no damage to the spermatogenesis of male recipient mice at the molecular level.

CLC Number: