Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (31): 5801-5804.doi: 10.3969/j.issn.1673-8225.2011.31.024

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HLA-A, B and DR high-resolution gene polymorphism of Henan Stem Cell Registry 

Wang Zhao-fu, Zhang Bo-wei, Ma Ru, Du Juan, Kong Da-wei   

  1. Henan Red Cross Blood Center, Zhengzhou   450012, Henan Province, China
  • Received:2011-02-11 Revised:2011-03-14 Online:2011-07-30 Published:2011-07-30
  • Contact: Zhang Bo-wei, Chief technician, Henan Red Cross Blood Center, Zhengzhou 450012, Henan Province, China
  • About author:Wang Zhao-fu, Associate professor, Henan Red Cross Blood Center, Zhengzhou 450012, Henan Province, China 659014888w@sina. com

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Abstract:

BACKGROUND: Since 2009, Chinese Marrow Donor Program takes more efforts in high resolution HLA typing, shortens typing retrieval time and increases the success rate of HLA typing.
OBJECTIVE: To statistically analyze HLA-A, B, DRB1 high-resolution gene polymorphism in bone marrow donors from Henan Province, China.
METHODS: A total of 3 874 venous blood samples were collected from the volunteer donors and subjected to ethylenediamine tetraacetic acid anticoagulation. The blood samples were detected using PCR-SSO HD high-resolution HLA typing method (LABType ® HD, Onelambda USA). The SSP and SBT detection tests were performed to solve ambiguity. Allele frequency was calculated with the direct count method.
RESULTS AND CONCLUSION: A total of 168 alleles were detected, 34 of which were for HLA-A, 84 for B, and 50 for DRB1. The top five loci for each was A*0201(0.160 9), A*1101 (0.162 7), A*2402(0.160 2), A*3001(0.080 8), A*3303 (0.078 9); B*1302(0.077 7), B*4001(0.072 2), B*5101(0.062 8), B*4601(0.061 2), B*0702(0.050 7); DRB1*1501(0.146 9), DRB1*0901(0.131 8 ), DRB1*0701(0.121 1), DRB1*1202(0.061 2), DRB1*0405(0.058 2).

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