Abstract:

BACKGROUND: The studies have proved that bone marrow mesenchymal stem cells transplantation therapy can improve or even reverse the liver fibrosis, but its mechanism is still unclear.
OBJECTIVE: To detect the effect of human bone marrow mesenchymal stem cells culture supernatant on the expression of matrix metalloproteinase 2 and tissue inhibitor of metalloproteinase 1 in hepatic stellate cells.
METHODS: The density gradient centrifugation was used to isolate human bone marrow mesenchymal stem cells and collected the culture supernatant of human bone marrow mesenchymal stem cells after cultured for 7 days, the human bone marrow mesenchymal stem cells culture supernatant added with the hepatic stellate cells was considered as experimental group, a simple hepatic stellate cells group and simple human bone marrow mesenchymal stem cells group were set up as control groups. The expressions of matrix metalloproteinase-2 protein and tissue inhibitor of matrix metalloproteinase-1 in the culture supernatant of three groups were detected after cultured for 24 and 48 hours.
RESULTS AND CONCLUSION: The expression of matrix metalloproteinase-2 protein and tissue inhibitor of matrix metalloproteinase-1 in the experimental group was decreased when compared with simple hepatic stellate cells group and simple human bone marnow mesenchymal stem cells group (P < 0.05 or P < 0.01). The results showed that bone marrow mesenchymal stem cells culture supernatant can inhibit the expression of matrix metalloproteinase-2 protein and tissue inhibitor of matrix metalloproteinase-1 in hepatic stellate cells.