Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (13): 2299-2303.doi: 10.3969/j.issn.1673-8225.2011.13.006

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Toll-like receptor 2 and 4 expression in peripheral blood leukocytes of patients following prosthesis implantation 

Zhang Zi-ji1, Kang Yan1, Zhai Qi-yi1, Zhang Hao1, Zhang Yang-chun1, Hou Chang-he1, Li Zi-qing1, Sheng Pu-yi1,2, Liao Wei-ming1    

  1. 1Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China
    2Huangpu Joint Surgery Center, First Affiliated Hospital of Sun Yat-sen University, Guangzhou  510700, Guangdong Province, China 
  • Received:2010-09-19 Revised:2010-10-20 Online:2011-03-26 Published:2011-03-26
  • Contact: Sheng Pu-yi, Doctor, Associate professor, Associate chief physician, Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China; Huangpu Joint Surgery Center, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510700, Guangdong Province, China shengpuyi@hotmail. com
  • About author:Zhang Zi-ji☆, Studying for doctorate, Physician, Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China zhangziji1984@sina.com
  • Supported by:

    the International Cooperation Program of Guangdong Provincial Science and Technology Commission, No. 2006B50107004*; the Science and Technology Development Program of Guangdong Provincial Science and Technology Commission, No. 2007B010600048*; the Scientific Research Foundation Program of University of Tampere, Finland, No. 9J114*

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Abstract:

BACKGROUND: Toll-like receptors (TLRs) are expressed on the surface of biofilms in loosening hip prosthesis. However, the expression changes remain unclear.
OBJECTIVE: To investigate the expression of TLR 2 and 4 in leukocytes of patients undergoing joint prosthesis implantation.
METHODS: Fasting blood plasma samples were harvested from elbow veins 24 hours post admission and 3 days post-operation from patients undergoing arthroplasty or arthroscopy (n=10). Cell-associated TLR 2 and 4 was measured by flow cytometry, white blood cells, C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) were tested.
RESULTS AND CONCLUSION: WBC, ESR, CRP were at normal level in both groups. TLR2 and 4 were mostly expressed in monocytes of peripheral blood, with low expression levels in lymphocytes and neutrophils. The expression levels of TLR2 was significantly reduced following operation (P < 0.05), but no significant difference was observed in the expression levels of TLR4 in arthroplasty group or TLR2 and 4 in arthroscopy group between post-operation and pre-operation (P > 0.05), whereas a significant change of TLR9 expression gap was found in arthroplasty patients before and after the procedure (P < 0.05). TLR 4 expression remained unchanged (P > 0.05). The TLR2 positive expression rate in monocytes of peripheral blood was significantly lower in arthroplasty group compared with arthroscopy group (P < 0.05), but no difference was found in TLR4 expression. Results show that in the absence of clinical infection, stress and tissue damage during arthroplasty and arthroscopy do not affect TLR2 and 4 expression, but TLR2 expression was downregulated following implantation of joint prosthesis.

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