Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (3): 405-409.doi: 10.3969/j.issn.1673-8225.2010.03.007

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Cytocompatibility of nano-hydroxyapatite/bacterial cellulose nanocomposites and its residues

Zheng Qi 1,2, Xi Ting-fei 1,2,3 , Chen Yan-mei3, Wang Zhao-xu2, Wan Yi-zao4, Gao Chuan4　   

  1. 1 School of Information & Engineering, Wenzhou Medical College, Wenzhou  325035, Zhejiang Province, China; 2 National Institute for Control of Pharmaceutical and Biological Products, Beijing   100050, China; 3 Academy for Advanced Interdisciplinary Studies, Peking University, Beijing   100871, China; 4 School of Materials Science and Engineering, Tianjin University, Tianjin  300072, China
  • Online:2010-01-15 Published:2010-01-15
  • Contact: Xi Ting-fei, Researcher, School of Information & Engineering, Wenzhou Medical College, Wenzhou 325035, Zhejiang Province, China; Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; National Institute for Control of Pharmaceutical and Biological Products, Beijing 100050, China Xiting-fei@tom.com
  • About author:Zheng Qi★, Studying for master’s degree, School of Information & Engineering, Wenzhou Medical College, Wenzhou 325035, Zhejiang Province, China; National Institute for Control of Pharmaceutical and Biological Products, Beijing 100050, China Flyangle2002@163.com
  • Supported by:

    the National 973 Plan Project of China, No. 2007CB936101; the   National 863 Plan Project of China, No. 2007AA021901

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Abstract:

BACKGROUND: The nano-hydroxyapatite/bacterial cellulose (nHAP/BC) nanocomposites has a good prospect of application in bone tissue engineering, and the bone tissue engineered materials and its degradation products should have excellent compatibility. This study further assessed DAN synthesis cycle using flow cytometry on the basis of evaluating cell compatibility by metabolic 3-( 4, 5-dim ethylthiazo l-2-y l) -2, 5-Dipheny l-2H-tetrazolium (MTT) assay.
OBJECTIVE: To evaluate the cytocompatibility of a new-pattern nHAP/BC nanocomposites and its residues. 
METHODS: Effects of nHAP/BC nanocomposites and its residues on morphological changes in osteoblasts were observed using in vitro cell culture method. Effects of nHAP/BC nanocomposites and its residues on osteoblast growth and proliferation were evaluated by MTT assay. Cell cycle phase changes were detected using flow cytometry to evaluate material effects on cell proliferation on molecular levels.
RESULTS AND CONCLUSION: The nHAP/BC nanocomposites and its residues had neither remarkable effects on cell morphology, nor significant inhibition on osteoblast growth and proliferation. Test of MTT cytotoxicity showed that the average cell proliferation rate was over 80% after treated with the material and its residues, with the cytotoxity grade of 1 (non-toxic). Flow cytometry indicated that the rate of G0/G1 was reduced, and the rates of S, G2/M were increased, and the synthesis of DNA was increased, the cellular growth and repair in osteoblasts was accelerated. These indicated that nHAP/BC nanocomposites have good cytocompatibility, and it will be safe and prospected scaffolds in bone tissue engineering.

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