Abstract:

BACKGROUND: Piperine is the lead compound of Rhodobryum roseum Limp that have protective effect on heart. Simultaneously, the chemical constitution of piperine is relative simple, which can provide a foundation for the further study of anti-myocardial ischemia.   
OBJECTIVE: To investigate the protective effect of piperine and its seven derivates on cardiocytes injured by isoproterenol or hydrogen peroxide (H2O2). 
METHODS: Injured cardiocyte model was induced by isoproterenol and H2O2. And then the influence of piperine and its seven derivates on injured cardiocytes was detected by MTT. 
RESULTS AND CONCLUSION: The 7×10^-8 mol/L piperine, 7×10^-8 , 7×10^-7 mol/L methyl piperate, 7×10^-8, 7×10^-7 , 7×10^-6 mol/L 5-(3, 4-methylenedioxy phenyl)-2E, 4E-pentadienoic acid pyrrolidine amide and 7×10^-8, 7×10^-7, 7×10^-6 mol/L 5-(3, 4- methylenedioxy phenyl)-2E, 4E-pentadienoic acid diethyl amide could significantly increased the survival of cardiocytes in the isoproterenol model group (P < 0.05), but there was no dose-dependent manner. And the survival of cardiocytes was also significantly higher in the H₂O₂ model group under the effect of 7×10-8mol/L piperine, 7×10^-8, 7×10^-7 mol/L 5-(3, 4-methylenedioxy phenyl)-2E, 4E-pentadienoic acid pyrrolidine amide and 7×10^-8, 7×10^-7 mol/L 5-(3.4-methylenedioxy phenyl)-2E, 4E-pentadienoic acid diethyl amide. In a word, piperine and its derivates could protect the primary cultured cardiocytes from isoproterenol damage and H2O2 damage. 5-(3, 4-methylenedioxy phenyl)-2E, 4E-pentadienoic acid pyrrolidine amide and 5-(3, 4-methylenedioxy phenyl)-2E, 4E-pentadienoic acid diethyl amide showed better protective effect than piperine.