Chemotaxis of vascular endothelial progenitor cells towards rat skin wounds following allogeneic transplantation

doi:10.3969/j.issn.2095-4344.2013.05.016

Abstract

Abstract:

BACKGROUND: It becomes a hotspot that allogeneic endothelial progenitor cells participate in vascular remodeling in ischemic tissues of the body.
OBJECTIVE: To observe the chemotaxis of allogeneic transplantation of vascular endothelial progenitor cells to rat skin wounds.
METHODS: Endothelial progenitor cells isolated from the peripheral blood of rats were cultured and labeled with BrdU, and then injected into rats with cutting injury on the back skin via the tail vein. Another normal rats served as controls. The number of BrdU positive endothelial progenitor cells in skin wound areas wasdetected by immunofluorescence staining at 1, 3, 7, 14 days after transplantation, and the changes in the number and volume of spleen lymphoid follicles were observed by hematoxylin-eosin staining.
RESULTS AND CONCLUSION: Endothelial progenitor cells could move to the injured skin areas. The number of endothelial progenitor cells was most at 3 and 7 days (P < 0.05), and BrdU positive cells could be seen until the 14th day in the vessel wall of the injured skin area. There was no significant difference between the two groups in the number and volume of spleen lymphoid follicles. No significant immunological rejection was caused by the allogeneic transplantation of vascular endothelial progenitor cells.

Key words: Organ transplantation, tissue transplantation, vascular endothelial progenitor cells, transplantation, skin injury, rat spleen, immune rejection, allogeneic transplantation, other grants-supported paper

CLC Number:

R318

Liu Chao, Zhao Ji-lin, Miao Lei-ying. Chemotaxis of vascular endothelial progenitor cells towards rat skin wounds following allogeneic transplantation[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(5): 866-871.

Figures/Tables 8

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