Spatiotemporal expression of bone morphologic protein 7 in mouse kidney development

doi:10.12307/2026.036

Abstract

Abstract: BACKGROUND: Bone morphogenetic protein 7 is mainly synthesized in the kidney and is expressed at different stages of kidney development, but the spatiotemporal distribution of its expression and its relationship with kidney development have not been systematically reported.
OBJECTIVE: To observe the expression of bone morphogenetic protein 7 in mice kidneys during different developmental stages and to explore its potential relationship with kidney development.
METHODS: Immunohistochemical techniques combined with stereological methods and western blot analysis were used to observe and analyze the expression of bone morphogenetic protein 7 in mouse kidney tissues at embryonic days (E)12, 14, 16, 18, and neonatal days (N)1, 3, 7, 14, 24, 40, and 70.
RESULTS AND CONCLUSION: (1) The results of immunohistochemistry showed that bone morphogenetic protein 7 was mainly expressed in the nephrogenic zone and immature renal corpuscles during the early stage of kidney development. Subsequently, it was located in mature renal corpuscles and the surrounding distal convoluted tubules, and the expression range gradually increased. After N40 days, bone morphogenetic protein 7 positively expressed in the renal interstitium. (2) Stereology and western blot results showed that the expression level of bone morphogenetic protein 7 gradually decreased with the development of the kidney, reached the lowest level at N7 days, and then slowly increased. To conclude, during the process of kidney development, the expression of bone morphogenetic protein 7 has significant spatiotemporal specificity, and it is hypothesized that it may be related to the development and maturation of renal corpuscles and distal convoluted tubules.

Key words: bone morphogenetic protein 7, kidney, development, mouse, renal corpuscle, distal convoluted tubule, engineered tissue construction

CLC Number:

Sun Yajie, Zhao Xinchen, Bo Shuangling. Spatiotemporal expression of bone morphologic protein 7 in mouse kidney development[J]. Chinese Journal of Tissue Engineering Research, 2026, 30(5): 1156-1161.

Figures/Tables 3

2.1 免疫组织化学结果 BMP-7阳性表达部位表现为棕褐色。E12 d时，输尿管芽分支出现，周围为密集的生后肾组织，可见BMP-7在输尿管芽和生后肾组织均有表达，见图1A。E14 d时，帽状生后肾组织逐渐发育为逗号小体、S小体和Ⅲ期肾小体，BMP-7不仅表达于输尿管芽的基底膜，而且在各期肾小体均可见表达，见图1B。E16 d时，第1代髓旁肾单位出现，BMP-7阳性表达和E14 d相似。E18 d时，Ⅲ期、Ⅳ期肾小体以及发育中的远曲小管中均可见BMP-7强阳性表达，见图1C。N1 d-N3 d时，随着被膜下生肾区逐渐变薄，髓旁肾小体数量增加，BMP-7在髓旁肾小体和远曲小管呈阳性表达，但是在髓质和髓放线中均未见阳性表达，见图1D和图1E。N7 d时，皮质生肾区消失，肾小体的数量趋于稳定，BMP-7的阳性表达集中在肾小体及其周围的部分远曲小管中，见图1F。N14 d-N40 d时，肾脏体积增大，皮质增厚，肾小体中BMP-7的表达逐渐减弱，同时肾小管逐渐发育完善，BMP-7在远曲小管的表达范围增加，但在远直小管、细段和近端小管中均未见BMP-7表达，见图1G和图1H。N70 d时，肾小体仍有BMP-7表达，且肾脏间质中也可见表达，见图1I。 2.2 体视学测量结果从E12 d到N7 d随着小鼠肾脏的发生发育，BMP-7在肾组织内表达的体密度值逐渐降低，E14 d、E18 d、N1 d、N3 d、N7 d的体密度值与前一组比较均差异显著，随后体密度值缓慢升高，N14 d、N70 d的体密度值与前一组比较差异显著(P < 0.05，图2)。 2.3 蛋白免疫印迹结果以GAPDH为内参，应用Iamage J软件对条带进行半定量分析。BMP-7在胚胎时期含量较多，随后逐渐下降，在N7 d达到最低，N14 d-N40 d趋于稳定。N70 d时，BMP-7表达略有增高。经统计学分析，组间数值差异显著(P < 0.05，图3)。"

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