Abstract: BACKGROUND: Neuregulin 1 has been shown to be characterized in cell proliferation, differentiation, and vascular growth. Human amniotic mesenchymal stem cells are important seed cells in the field of tissue engineering, and have been shown to be involved in tissue repair and regeneration.
OBJECTIVE: To construct human amniotic mesenchymal stem cells overexpressing neuregulin 1 and investigate their proliferation and migration abilities, as well as their effects on wound healing.
METHODS: (1) Human amniotic mesenchymal stem cells were in vitro isolated and cultured and identified. (2) A lentivirus overexpressing neuregulin 1 was constructed. Human amniotic mesenchymal stem cells were divided into empty group, neuregulin 1 group, and control group, and transfected with empty lentivirus and lentivirus overexpressing neuregulin 1, or not transfected, respectively. (3) Edu assay was used to detect the proliferation ability of the cells of each group, and Transwell assay was used to detect the migration ability of the cells. (4) The C57 BL/6 mouse trauma models were constructed and randomly divided into control group, empty group, neuregulin 1 group, with 8 mice in each group. Human amniotic mesenchymal stem cells transfected with empty lentivirus or lentivirus overexpressing neuregulin-1 were uniformly injected with 1 mL at multiple local wound sites. The control group was injected with an equal amount of saline. (5) The healing of the trauma was observed at 1, 7, and 14 days after model establishment. Histological changes of the healing of the trauma were observed by hematoxylin-eosin staining. The expression of CD31 on the trauma was observed by immunohistochemistry. 
RESULTS AND CONCLUSION: (1) Human amniotic mesenchymal stem cells overexpressing neuregulin-1 were successfully constructed. The mRNA and protein expression of intracellular neuregulin 1 was significantly up-regulated compared with the empty group (P < 0.05). (2) The overexpression of neuregulin 1 promoted the migratory ability (P < 0.01) and proliferative ability of human amniotic mesenchymal stem cells (P < 0.05). (3) Human amniotic mesenchymal stem cells overexpressing neuregulin 1 promoted wound healing in mice (P < 0.05) and wound angiogenesis (P < 0.05). The results showed that overexpression of neuregulin 1 resulted in an increase in the proliferative and migratory capacities of human amniotic mesenchymal stem cells, significantly promoting wound healing and angiogenesis.

Key words: human amniotic mesenchymal stem cell, neuregulin-1, overexpression, cell proliferation, cell migration, mouse, wound, healing ,