Effects of Biodentine on proliferation and osteogenesis of MG-63 cells

doi:10.3969/j.issn.2095-4344.0776

Abstract

Abstract:

BACKGROUND: As a bioactive substance, Biodentine has good compressive strength, bonding strength and less micro-leakage. It has been successfully applied to a variety of clinical indications. However, much less is known about whether Biodentine can promote osteogenesis.

OBJECTIVE: To explore the effects of Biodentine of different concentrations on proliferation and osteogenesis of MG-63 cells.

METHODS: The extracts of Biodentine with different concentration gradients (1, 1/2, 1/4, 1/8, 1/16) were prepared. MG-63 cells were cultured in six groups, with the addition of minimum essential medium (control group) and five concentrations of Biodentine extracts. Cell proliferation was detected by cell counting kit-8 assay at 1, 3, 5 and 7 days, and then the best concentration of Biodentine extract was screened. Another MG-63 cells were cultured in minimum essential medium (blank control group) and Biodentine extract of the optimal concentration (experimental group). The expression of osteogenic factor Runx2 mRNA in MG-63 cells was detected by real-time PCR at 1, 3, 5 and 7 days of culture. Then alizarin red staining was used to observe calcified nodules in MG-63 cells at 10 and 14 days of culture.

RESULTS AND CONCLUSION: (1) At 1 and 3 days of culture, the number of viable cells in different concentration groups was similar to that in the control group (P > 0.05). At 5 days of culture, compared with the control group, the number of viable cells in MG-63 cells was significantly lowered in the 1 concentration group, showed no significant changes in the 1/2, 1/4, 1/8 concentration groups (P > 0.05), and was significantly increased in the 1/16 concentration group (P < 0.05). Therefore, the 1/16 concentration of Biodentine extract was used for further experiment. (2) Runx2 mRNA expression of the experimental group at 1, 3, 5 and 7 days of culture was 1.14, 5.29, 1.08 and 2.11 times that of the control group, respectively (all P < 0.05). (3) At 10 days of culture, both the experimental group and blank control group showed no mineralized nodules; at 14 days of culture, mineralized nodules were observed in the two groups, and larger and darker nodules were seen in the experiment group. To conclude, Biodentine at certain concentrations can promote the proliferation and osteogenic activity of human osteosarcoma cell line MG-63.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

CLC Number:

R318

Zhai Jiao-yan, Wang Li-na, Liu Qi-cheng, Shi Chun, Niu Wei-dong. Effects of Biodentine on proliferation and osteogenesis of MG-63 cells[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(18): 2843-2848.

Figures/Tables 4

References

[1] De Rossi A,Fukada SY,De Rossi M,et al.Cementocytes Express Receptor Activator of the Nuclear Factor Kappa-B Ligand in Response to Endodontic Infection in Mice.J Endod. 2016;42(8):1251-1257.

[2] Kok SH,Hou KL, Hong CY,et al.Sirtuin 6 Modulates Hypoxia-induced Apoptosis in Osteoblasts via Inhibition of Glycolysis:Implication for Pathogenesis of Periapical Lesions. J Endod. 2015;41(10): 1631-1637.

[3] Zhang R,Huang S,Wang L,et al.Histochemical Localization of Dickkopf-1 in Induced Rat Periapical Lesions.J Endod. 2014;40(9): 1394-1399.

[4] Parirokh M,Torabinejad M,Dummer PMH.Mineral trioxide aggregate and other bioactive endodontic cements: An updated overview- Part I: Vital pulp therapy.Int Endod J.2017; 24.doi: 10.1111/iej.12841.

[5] Huang TH,Ding SJ,Hsu TC,et al.Effects of mineral trioxide aggregate (MTA) extracts on mitogen-activated protein kinase activity in human osteosarcoma cell line(U2OS). Biomaterials. 2003;24 (22):3909-3913.

[6] Huang TH,Yang CC,Ding SJ,et al.Inflammatory cytokines reaction elicited by root-end filling materials.J Biomed Mater Res B Appl Biomater.2005;73(1):123-128.

[7] Bruserud O,Wendelbo O,Paulsen K.Lipoteichoic acid derived from Enterococcus faecalis modulates the functional characteristics of both normal peripheral blood leukocytes and native human acute myelogenous leukemia blasts.Eur J Haematol.2004;73(5):340-350.

[8] Maeda H,Nakano T,Tomokiyo A,et al. Mineral Trioxide Aggregate induces bone morphogenetic protein-2 expression and calcification in human periodontal ligament cells.J Endod. 2010;36(4):647-652.

[9] Gomes-Filho JE,Rodrigues G,Watanabe S,et al.Evaluation of the tissue reaction to fast endodontic cement (CER) and Angelus MTA.J Endod.2009;35(10):1377-1380.

[10] Camilleri J.Characterization and hydrationkinetics of tricalciumsilicatecement for use as a dentalbiomaterial.Dent Mater. 2011;27(8):836-844.

[11] Malkondu Ö,Karapinar Kazanda? M,Kazazo?lu E.A Review on Biodentine, a Contemporary Dentine Replacement and Repair Material.Biomed Res Int.2014;2014:160951.

[12] Grech L,Mallia B,Camilleri J.Characterization of set Intermediate Restorative Material, Biodentine, Bioaggregate and a prototype calcium silicate cement for use as root-end filling materials. Int Endod J.2013;46(7):632-641.

[13] Kayahan MB,Nekoofar MH,McCann A,et al.Effect of acid etching procedures on the compressive strength of 4 calcium silicate-based endodontic cements.J Endod. 2013;39(12):1646-1648.

[14] Grech L,Mallia B,Camilleri J.Investigation of the physical properties of tricalcium silicate cement-based root-end filling materials.Dent Mater. 2013;29(2):e20-28.

[15] Koubi G,Colon P,Franquin JC,et al.Clinical evaluation of the performance and safety of a new dentine substitute, Biodentine, in the restoration of posterior teeth—a prospective study. Clin Oral Investig. 2013;17(1):243-249.

[16] Guneser MB,Akbulut MB,Eldeniz AU.Effect of various endodontic irrigants on the push-out bond strength of biodentine and conventional root perforation repair materials. J Endod.2013;39(3): 380-384.

[17] Camilleri J,Grech L,Galea K,et al.Porosity and root dentine to material interface assessment of calcium silicate-based root-end filling materials.Clin Oral Investig. 2014;18(5):1437-1446.

[18] De Souza ET,Nunes Tameirão MD,Roter JM,et al.Tridimensional quantitative porosity characterization of three set calcium silicate-based repair cements for endodontic use.Microsc Res Tech. 2013;76(10): 1093-1098.

[19] Camilleri J,Sorrentino F,Damidot D.Investigation of the hydration and bioactivity of radiopacified tricalcium silicate cement,Biodentine and MTA Angelus.Dent Mater. 2013;29(5):580-593.

[20] Tanalp J,Karap?nar-Kazanda? M,Döleko?lu S,et al.Comparison of the radiopacities of different root-end filling and repair materials. Scientific World J.2013;23:594950.

[21] Raskin A,Eschrich G,Dejou J,et al.In vitro microleakage of Biodentine as a dentin substitute compared to Fuji II LC in cervical lining restorations.J Adhes Dent.2012;14(6):535-542.

[22] Villat C,Tran XV,Pradelle-Plasse N,et al.Impedance methodology: A new way to characterize the setting reaction of dental cements.Dent Mater.2010;26(12):1127-1132.

[23] Vallés M,Roig M,Duran-Sindreu F,et al.Color Stability of Teeth Restored with Biodentine: A 6-month In Vitro Study.J Endod. 2015; 41(7):1157-1160.

[24] Vallés M,Mercadé M,Duran-Sindreu F,et al.Influence of light and oxygen on the color stability of five calcium silicate-based materials.J Endod.2013;39(4):525-528.

[25] Han L,Okiji T.Bioactivity evaluation of three calcium silicate-based endodontic materials.Int Endod J. 2013;46(9):808-814.

[26] Bortoluzzi EA,Broon NJ,Bramante CM,et al.The influence of calciumchloride on the setting time, solubility, disintegration, and pH of mineral trioxide aggregate and white Portland cementwith a radiopacifier. J Endod.2009;35(4):550-554.

[27] Villat C,Tran VX,Pradelle-Plasse N,et al.Impedance methodology: a new way to characterize the setting reaction of dental cements.Dent Mater.2010;26(12):1127-1132.

[28] Nowicka A,Lipski M,Parafiniuk M,et al.Response of human dental pulp capped with biodentine and mineral trioxide aggregate.J Endod. 2013; 39(6):743-747.

[29] Shayegan A,Jurysta C,Atash R,et al.Biodentine used as a pulp-capping agent in primary pig teeth. Pediatr Dent.2012;34(7):e202-208.

[30] Zanini M,Sautier JM,Berdal A,et al.Biodentine induces immortalized murine pulp cell differentiation into odontoblast-like cells and stimulates biomineralization.J Endod.2012;38(9):1220-1226.

[31] Girish K,Mandava J,Chandra RR,et al.Effect of obturating materials on fracture resistance of simulated immature teeth.J Conserv Dent. 2017; 20(2):115-119.

[32] Villat C,Grosgogeat B,Seux D,et al.Conservative approach of a symptomatic carious immature permanent tooth using a tricalcium silicatecement (Biodentine): a case report.Restor Dent Endod. 2013; 38(4):258-262.

[33] Pawar AM,Kokate SR,Shah RA.Management of a large periapical lesion using Biodentine as retrograde restoration with eighteen months evident follow-up.J Conserv Dent. 2013;16(6):573-575.

[34] Bakhtiar H,Nekoofar MH,Aminishakib P,et al.Human Pulp Responses to Partial Pulpotomy Treatment with TheraCal as Compared withBiodentine and ProRoot MTA: A Clinical Trial.J Endod.2017;16.pii: S0099-2399(17)30812-9.

[35] Goel S,Nawal RR,Talwar S.Management of Dens Invaginatus Type II Associated with Immature Apex and Large Periradicular Lesion Using Platelet-rich Fibrin and Biodentine. J Endod.2017;13.pii: S0099-2399(17)30417-X.

[36] Jalan AL,Warhadpande MM,Dakshindas DM.A comparison of human dental pulp response to calcium hydroxide and Biodentine as direct pulp-capping agents.J Conserv Dent. 2017;20(2):129-133.

[37] Zanini M,Sautier JM,Berdal A,et al.Biodentine induces immortalized murine pulp cell differentiation into odontoblast-like cells and stimulates biomineralization.J Endod.2012;38(9): 1220-1226.

[38] Luo Z,Li D,Kohli MR,et al.Effect of Biodentine on the proliferation, migration and adhesion of human dental pulp stem cells.J Dent. 2014;42(4):490-497.

[39] Zhou HM,Shen Y,Wang ZJ,et al.Haapasalo. In vitro cytotoxicity evaluation of a novel root repair material.J Endod. 2013;39(4):478-483.

[40] Luo Z,Kohli MR,Yu Q,et al.Biodentine induces human dental pulp stem cell differentiation through mitogen-activated protein kinase and calcium-/calmodulin-dependent protein kinase II pathways.J Endod. 2014;40(7):937-942.

[41] Ho CC,Fang HY,Wang B,et al.The effects of Biodentine/polycaprolactone three-dimensional -scaffold with odontogenesis properties on human dental pulp cells. Int Endod J. 2017; 20.doi: 10.1111/iej.12799.

[42] Laurent P,Camps J,About I.Biodentine(TM) induces TGF-beta1 release from human pulp cells and early dental pulp mineralization.Int Endod J.2012;45(5):439-448.

[43] Jung JY,Woo SM,Lee BN,et al. Effect of Biodentine and Bioaggregate on odontoblastic differentiation via mitogen-activated protein kinase pathway in human dental pulp cells.Int Endod J.2015;48(2): 177-184.

[44] Chang SW,Lee SY,Ann HJ,et al.Effects of calcium silicate endodontic cements on biocompatibility and mineralization-inducing potentials in human dental pulp cells.J Endod.2014;40(8):1194-1200.

[45] Perard M,Le Clerc J,Watrin T,et al.Spheroid model study comparing the biocompatibility of Biodentine and MTA.J Mater Sci Mater Med. 2013;24(6):1527-1534.

[46] Corral Nuñez CM,Bosomworth HJ,Field C,et al.Biodentine and mineral trioxide aggregate induce similar cellular responses in a fibroblast cell line.J Endod. 2014;40(3):406-411.

[47] Jang YE,Lee BN,Koh JT,et al.Cytotoxicity and physical properties of tricalcium silicate-based endodontic materials. Restor Dent Endod. 2014;39(2):89-94.

[48] Poggio C,Arciola CR,Beltrami R,et al.Cytocompatibility and antibacterial properties of capping materials. ScientificWorldJournal. 2014;2014:181945.

[49] Poggio C,Ceci M,Beltrami R,et al. Biocompatibility of a new pulp capping cement.Ann Stomatol (Roma).2014;5(2):69-76.

[50] Lee BN,Lee KN,Koh JT,et al.Effects of 3 endodontic bioactive cements on osteogenic differentiation in mesenchymal stem cells.J Endod. 2014;40(8):1217-1222.