Neuroprotective effects of sufentanil preconditioning on spinal cord injury in mouse models

doi:10.3969/j.issn.2095-4344.2016.40.006

Abstract

Abstract:

BACKGROUND: Sufentanil exerts protective effects on tissues, but its roles in the repair of nervous system injury and the underlying mechanism are still unknown.

OBJECTIVE: To explore the protective effect of sufentanil preconditioning in the repair of spinal cord injuries and the underlying mechanism.

METHODS: Mouse models of spinal cord injuries were prepared through clipping spinal cord followed by intraperitoneal injection of 3 and 6 μg/kg sufentanil, respectively.

RESULTS AND CONCLUISON: (1) Western blotting, ELISA and TUNEL assays showed that 6 μg/kg sufentanil significantly down-regulated the protein expression levels of TLR4, nuclear factor-κBp65, cleaved-caspase 3, tumor necrosis factor-α and interlenkin-1β in the spinal cord of mice (P < 0.05); at the same time, the number of apoptotic neurons was significantly decreased (P < 0.05). (2) Furthermore, high-dose sufentanil preconditioning significantly ameliorated the recovery of limb function at 14 days after injury (P < 0.05). (3) These results administrate that the neuroprotection provided by 6 μg/kg sufentanil preconditioning for spinal cord injuries in mice maybe related to the TLR4/nuclear factor-κB signaling pathway inactivation.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Tissue Engineering, Spinal Cord Injuries, Models, Animal

CLC Number:

R318

Yu Yu-qin, Hu Nian-chun, Duan Ji-an, Li Da-peng, Liu Chang. Neuroprotective effects of sufentanil preconditioning on spinal cord injury in mouse models[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(40): 5966-5972.

Figures/Tables 2

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