Expression of interleukin-10 and cyclooxygenase 2 in keratitis rat models

doi:10.3969/j.issn.2095-4344.2015.49.025

Abstract

Abstract:

BACKGROUND: Investigating the relationship between interleukin-10, cyclooxygenase-2 expression levels and the occurrence and development of keratitis is of great significance for the treatment of keratitis.
OBJECTIVE: To detect the expression of interleukin-10 and cyclooxygenase 2 in keratitis rat models and analyze the relationship between interleukin-10, cyclooxygenase-2 expression and the occurrence and development of keratitis in rats.
METHODS: Twenty-six healthy rats of either gender were randomly selected to establish keratitis rat models. The left eyes of rats were chosen as experimental group to establish keratitis models using epikeratophakia. The right eyes of rats were set as control group. At the 1th, 3rd, 7th and 14th days after modeling, the pathological changes of rat corneal tissue were observed in both eyes. The expression of interleukin-10 and cyclooxygenase 2 in the corneal tissue with different degrees of keratitis was determined using immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR).
RESULTS AND CONCLUSION: Histopathological observation showed that there were severe tissue necrosis and neutrophil infiltration in keratitis foci. PAS staining showed that the mycelium was mainly present in the corneal stroma. In the control group, the expression of interleukin-10 was hardly detected in rat cornea. After keratitis infection, the expression of interleukin 10 increased at first and then decreased, and there was significant difference between different time points (P < 0.01). In the control group, the expression of cyclooxygenase-2 was hardly detected in rat cornea. After keratitis infection, the expression of cyclooxygenase 2 was increased gradually and then decreased at the 14th day. There was significant difference between different time points (P <
0.01). PAS staining and pathologic tissue culture experiment suggest that rat models of keratitis were successfully established. These results show that the expression of interleukin-10 appeared in the late period of keratitis lesions, which indicate that interleukin-10 may be involved in the tissue damage repair in the late period of keratitis. The expression of cyclooxygenase-2 locates in the keratitis tissue and cyclooxygenase-2 is a sensitive inflammatory cytokine of keratitis.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

CLC Number:

R318

Lu Xia, Pan Xu-bin, Xie Yu-tao, Ji Qiu-di. Expression of interleukin-10 and cyclooxygenase 2 in keratitis rat models[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(49): 8015-8020.

Figures/Tables 8

References

[1] 李洪霞,张宏波,王宜强.T 和 B 细胞缺陷对真菌性角膜炎发病过程的影响[J].国际眼科杂志,2012,12(12):2247-2252.

[2] 苏晶,崔红平.烟曲霉菌性角膜炎大鼠角膜IL-6和IL-10的表达[J].眼科研究,2008,26(8):579-582.

[3] 王晓雪.IL-L与IL-10在大鼠真菌性角膜炎固有免疫阶段角膜上皮组织中的表达[D]. 青岛:青岛大学,2012.

[4] 苏晶.大鼠烟曲霉菌性角膜炎动物模型的建立及其免疫病理的研究[D].上海:同济大学医学院,2006.

[5] 刘筱楠.CXCL1在大鼠烟曲霉菌角膜炎早期的表达[D]. 青岛:青岛大学, 2012.

[6] Sellers RS, Silverman L, Khan KN. Cyclooxygenase-2 expression in the cornea of dogs with keratitis.Vet Pathol. 2004; 41(2):116-121.

[7] Li N, Che CY, Hu LT, et al.Effects of COX-2 inhibitor NS-398 on IL-10 expression in rat fungal keratitis.Int J Ophthalmol. 2011; 4(2):165-169.

[8] 张明昌,王勇.角膜碱烧伤中白介素-1与核转录因子κB表达的相关性研究[J].眼科研究,2007,25(1):33-36.

[9] 梁丽娜,庄曾渊.带状疱疹病毒性角膜内皮炎合并瞳孔损害 2 例[J].中国中医眼科杂志,2013,2(1):30-31.

[10] 夏丽坤,张劲松,陈晓隆,等.HSV-1上调白细胞介素-18 mRNA在小鼠角膜组织中表达的研究[J].眼科研究,2005,23(4):393-396.

[11] 夏丽坤,高殿文,濮伟,等.白细胞介素10对小鼠单纯疱疹性角膜基质炎作用的实验研究[J].中华眼科杂志,2003,39(10):592-596.

[12] 林秀丽.兔棘阿米巴性角膜炎IL-1β和MCP-1的表达[D].福州:福建医科大学,2010.

[13] 曾宗圣,韩晓丽,胡建章,等.白细胞介素-17和维甲酸相关核孤儿受体γt在小鼠真菌性角膜炎中的表达[J].中华实验眼科杂志, 2013,31(7):653-658.

[14] Hazlett LD, Jiang X, McClellan SA.IL-10 function, regulation, and in bacterial keratitis.J Ocul Pharmacol Ther.2014;30(5): 373-380.

[15] Biswas PS, Banerjee K, Kim B,et al. Role of inflammatory cytokine-induced cyclooxygenase 2 in the ocular immunopathologic disease herpetic stromal karatitis. J Virol.2005;79(16):10589-10600.