Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (25): 3977-3982.doi: 10.3969/j.issn.2095-4344.2015.25.009

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Lyophilizing canine allogeneic mandible: meeting the requirements for rebuilding strength, shape and support capabilities  

Jing Cai-xia1, Liu Chang-kui2, Tan Xin-ying2, Hu Min2   

  1. 1Department of Pathogenic Biology, Medical College of Yan’an University, Yan’an 716000, Shaanxi Province, China; 
    2Department of Stomatology, General Hospital of Chinese PLA, Beijing 100853, China
  • Online:2015-06-18 Published:2015-06-18
  • Contact: Hu Min, Professor, Doctoral supervisor, Department of Stomatology, General Hospital of Chinese PLA, Beijing 100853, China
  • About author:Jing Cai-xia, Master, Lecturer, Department of Pathogenic Biology, Medical College of Yan’an University, Yan’an 716000, Shaanxi Province, China
  • Supported by:

     the National Natural Science Foundation of China, No. 81470726

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Abstract:

 

BACKGROUND: The favorable structure and biological characteristics of allogeneic temporomandibular joint become an effective solution for condylar defect, but immunologic rejection and slow ossification are the main problem for the presence of bone allograft.
OBJECTIVE: To meet the requirements of rebuilding mandible defect by lyophilizing canine mandible.
METHODS: The periosteum, soft tissue, and cartilage of 12 canine mandibles were removed. 1 mm diameter hole was drilled with 1 to 2 cm intervals in their cortical bones with a fissure bur. After washing, they were placed into a -4 ℃ refrigerator for 12 hours, and then stored at -80 ℃ gradually for 1 week. The mandibles were put in a drier. When the moisture content of osseous tissue decreased to 5%, the bones were packed in aseptic environment, radio pasteurized, and stored in vacuum container at atmosphere temperature. A biomechanical test was conducted after the lyophilization.

RESULTS AND CONCLUSION: The maximum shifts of lyophilized mandibles in compression and bending tests were slight according to the steep load-shift curve. The plastic zone was insignificant and fractures appeared immediately when the pressure exceeded the plastic zone. The maximum load, maximum shift, and rigidity in the compression test were (5 163.10±730.16) N, (0.78±0.19) mm and (11 069.17±1 758.12) N/mm, respectively. The data in bending test were (486.67±134.12) N, (0.67±0.15) mm and (1 151.67±256.46) N/mm, respectively. It is concluded that the dehydrated and lyophilized canine mandibles have good shape and support ability and can meet the mechanical requirements in repairing and reconstructing mandibular defect. 

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

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