Action potential and L-type calcium channel currents in myocardial cells of rat models of heroin addiction

doi:10.3969/j.issn.2095-4344.2015.18.010

Abstract

Abstract:

BACKGROUND: Calcium channel abnormalities can damage myocardium. Heroin can directly affect calcium ion channel, and alter myocardial structure.
OBJECTIVE: To study the changes of heroin-induced myocardial ultrastructure, L-type calcium channel current and action potential of myocardial cells after rat cardiac arrhythmia.
METHODS: Sprague-Dawley rats were randomly divided into control group and model group. In the model group, rats were administered heroin at initial dose of 5 mg/kg•d. The daily dose escalation method was used (increasing dose: 2.5 mg/kg•d) to replicate rat models of heroin addiction. At 20 days, models of heroin addiction were successfully established. At 30 days after increasing the dose, rat models of heroin addiction-induced arrhythmias were further established.
RESULTS AND CONCLUSION: Compared with the control group, the electron microscopy demonstrated that myocardial structure changes mainly presented nuclear membrane shrinkage, nuclear condensation, nuclei became small, chromatin assembled into blocks, mitochondria disordered and disappeared, sarcomeres disordered, focal fracture, and unclear myofilament in rat models of heroin addiction. Electric current-voltage curve of myocardial cell L-type calcium channel current showed the increasing trends. The 90% repolarization action potential was significantly shortened. These data indicated that heroin can directly lead to the pathological change of myocardial structure. Calcium channel current change is one of the main reasons for myocardial injury.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: Heroin Dependence, Myocytes, Cardiac, Arrhythmias, Cardiac, Calcium Channels, L-type

CLC Number:

R318

Pu Hong-wei, Su Li-ping, Wang Xue-mei, Chen Xiao, Zhang Li-ping, Liu Xiao-shan, Wang Zhi-guo, Wang Hua, Li Kai-chao. Action potential and L-type calcium channel currents in myocardial cells of rat models of heroin addiction[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(18): 2843-2848.

Figures/Tables 2

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