Metabolomic analysis of urine in a rat model of chronic myofascial trigger points

doi:10.12307/2025.351

Abstract

Abstract: BACKGROUND: Chronic myofascial trigger points can identify differential metabolite changes through non targeted metabolomics techniques, helping to understand and further explore the pathophysiological processes and pathogenesis of chronic myofascial trigger points from the perspective of endogenous small molecule metabolites.
OBJECTIVE: To investigate potential biomarkers and related metabolic pathways based on urine metabolomics in the rat model of chronic myofascial trigger points.
METHODS: Sixteen Sprague-Dawley rats were randomly divided into a model group and a normal group. The model group was used to establish a chronic myofascial trigger point animal model by combining blunt hitting with centrifugal exercise (treadmill slope: -16°, running speed: 16 m/min, training time: 90 minutes each), once a week for 8 continuous weeks, with 4 weeks off. After 12 weeks of modeling, the metabolic cage method was used to collect urine from rats at 24 hours after modeling. Liquid chromatography-mass spectrometry non-targeted metabolomics technology was used to detect metabolic profiles in the urine samples, screen common differential metabolites, and conduct bioinformatics analysis.
RESULTS AND CONCLUSION: Compared with the normal group, there were 32 differential metabolic markers in the model group, of which 21 were upregulated and 11 were downregulated. A total of 14 differential metabolites were identified as potential biomarkers based on the value of variable important in projection greater than 3. The enrichment analysis of the Kyoto Encyclopedia of Genes and Genomes indicated that the formation of chronic myofascial trigger points is closely related to metabolic pathways such as primary bile acid biosynthesis and arachidonic acid metabolism.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: myofascial trigger point, rat, urine, metabolomics, bioinformatics, biomarker, differential metabolite, metabolic cage method, sports injuries

CLC Number:

Liu Lin, Liu Shixuan, Lu Xinyue, Wang Kan. Metabolomic analysis of urine in a rat model of chronic myofascial trigger points [J]. Chinese Journal of Tissue Engineering Research, 2025, 29(8): 1585-1592.

Figures/Tables 7

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