Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (3): 359-363.doi: 10.3969/j.issn.2095-4344.2016.03.010

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Effect of brominated furanones on the formation of Staphylococcus epidermidis biofilm on the surface of polyvinyl chloride material

Cheng Peng1, Ye Lian-hua1, Huang Yun-chao1, Xu Geng2, Zhao Guang-qiang1, Guo Feng-li3,Qiu Liang-ting1
  

  1. 1 Department of Thoracic Surgery, Third Affiliated Hospital of Kunming Medical University, Yunnan Province Cancer Hospital, Kunming 650118, Yunnan Province, China
    2 Department of Thoracic and Cardiovascular Surgery, Heze Municipal Hospital, Heze 274031, Shandong Province, China
    3 Bacterium Room, Department of Clinical Laboratory, Third Affiliated Hospital of Kunming Medical University, Yunnan Province Cancer Hospital, Kunming 650118, Yunnan Province, China
  • Received:2015-11-03 Online:2016-01-15 Published:2016-01-15
  • Contact: Ye Lian-hua, M.D., Associate chief physician, Master’s supervisor, Department of Thoracic Surgery, Third Affiliated Hospital of Kunming Medical University, Kunming 650118, Yunnan Province, China Corresponding author: Huang Yun-chao, M.D., Professor, Chief physician, Doctoral supervisor, Department of Thoracic Surgery, Third Affiliated Hospital of Kunming Medical University, Kunming 650118, Yunnan Province, China
  • About author:Cheng Peng, Studying for master's degree, Department of Thoracic Surgery, Third Affiliated Hospital of Kunming Medical University, Kunming 650118, Yunnan Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81260228, 81460278; the Natural Science Foundation of Yunnan Province, China, No. 2013FZ276, 2014FA048; Yunnan Provincial High-level Personnel Training Fund, China, No. 2012HB032, D-201222

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Abstract:

BACKGROUND: Quorum sensing system plays a very important role in the formation of bacterial biofilm. Most of brominated furanones blocked the quorum sensing of bacteria after combining with the receptors of autoinductors in sensing system, however, some brominated furanones play a role of bacterial autoinductors and promote the formation of bacterial biofilm.
OBJECTIVE: To investigate the influence of different types of brominated furanones on the biofilm formation of Staphylococcus epidermidis on the surface of polyvinyl chloride materials.
METHODS: Polyvinyl chloride materials were divided into control group and furanone 1-3 groups. The polyvinyl chloride materials in the control group were soaked with ethanol for 5 minutes. Three kinds of brominated furanones with representative chemical structure were taken as furanone 1-3 groups which were respectively 3,4-dibromo-5-hydroxyl-furanone,4-bromo-5-(4-methoxypheny)-3-(methylamino)- furanone and 3,4-dibromo-5,5-dimethoxypheny-2(5H)-furanone. The surface coating of the polyvinyl chloride materials in these four groups all underwent modification and then were co-cultivated with Staphylococcus epidermidis together.
RESULTS AND CONCLUSION: Compared with the control group, Staphylococcus epidermidis bacterium community quantity on the surface of polyvinyl chloride material was smaller, and the thickness of bacterium biofilm in furanone-2 group was thinner. There was no obvious bacterial biofilm structure formation on the surface of polyvinyl chloride material at the 18th hour of culture. The Staphylococcus epidermidis bacterium community quantity and the thickness of bacterium biofilm in furanone 1 and furanone 3 groups were closer to the control group. These results show that the impact of different types of brominated furanones on biofilm formation of Staphylococcus epidermidis on the surface of polyvinyl chloride materials is different, among them, 4-bromo-5-(4-methoxypheny)-3-(methylamino)-furanone can inhibit the growth of Staphylococcus epidermidis and the bacterial biofilm formation on the surface of polyvinyl chloride materials.