Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (3): 352-358.doi: 10.3969/j.issn.2095-4344.2016.03.009

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Construction of animal models induced by platelet-rich fibrin

Xiao Qiong1, Sun Yong2, Chen Hong-liang2, Zhao Feng2, Dong Lu3, Yang Qin-qiu4, Lu Shuai2   

  1. 1Jiulongpo Dental Hospital of Chongqing Bybo Dental Hospital Administrative Limited Company, Chongqing 400000, China; 2Department of Stomatology, the Chengdu Military Region Authorities Hospital of PLA, Chengdu 610041, Sichuan Province, China; 3Department of Stomatology, the First People’s Hospital of Liangshan Yi Autonomous Prefecture, Xichang 615000, Sichuan Province, China; 4Department of Stomatology, the First People’s Hospital of Suining, Suining 629000，Sichuan Province, China
  • Received:2015-11-07 Online:2016-01-15 Published:2016-01-15
  • Contact: Sun Yong, Chief physician, Professor, Department of Stomatology, the Chengdu Military Region Authorities Hospital of PLA, Chengdu 610041, Sichuan Province, China
  • About author:Xiao Qiong, Master, Physician, Jiulongpo Dental Hospital of Chongqing Bybo Dental Hospital Administrative Limited Company, Chongqing 400000, China
  • Supported by:

    the Military “Twelfth Five-Year” Research Program, China, No. CWS11J024; the Key Laboratory Open Project of Militay Oral Medicine, No.2014KB04

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Abstract:

BACKGROUND: At present, many studies have focused on platelet-rich fibrin combined with other bone substitute materials in repair of peri-implant bone defects and sinus lifting, but there is still a lack of research 
about the platelet-rich fibrin alone in repair of critical-size bone defects.
OBJECTIVE: To compare the effect of three kinds of bone substitutes, platelet-rich fibrin, Bio-Oss bone substitute and autogenous cancellous bone, in repair of bone defects.
METHODS: Four areas of cylindrical critical bone defects with a diameter of 6.0 mm and depth of 10.0 mm were prepared in the medial femoral condyle of beagle dogs. Three areas of bone defects were implanted with autologous platelet-rich fibrin, Bio-Oss bone substitutes and autologous cancellous bone, respectively. The remaining one area of bone defect was not implanted any substance, as control group. X-ray and Micro-CT detections in bone defect area were conducted after 12 weeks of surgery.
RESULTS AND CONCLUSION: (1) X-ray: the density of platelet-rich fibrin group was more higher, but still slightly lower than that of the surrounding normal bone tissue; the density of the Bio-Oss bone substitute group was more lower, but still higher than that of the surrounding bone tissues; the density of autologous cancellous bone group was more higher, which was close to that of the surrounding bone tissues; circular low-density images were visible in the control group. (2) Micro-CT: platelet-rich fibrin group was similar with the autogenous cancellous bone group, and their density was slightly lower than that of the surrounding normal bone tissues; the trabecular bone exlibited a clear regular arrangement, and no obvious interface; the Bio-Oss bone substitute group showed a high density image with clear interface; the bone defect area was still clearly visible in the control group, with a low-density image. The bone volume fraction and trabecular number in the platelet-rich fibrin and autologous cancellous bone groups were both higher than those in the Bio-Oss bone substitute group (P < 0.05). These results demonstrate that platelet-rich fibrin and autogenous cancellous bone have a similar bone repair effect.