Dimethyl sulfoxide acts as a protective agent to perfuse rabbit amputated limbs: the relative recovery of local drug concentrations

doi:10.3969/j.issn.2095-4344.2015.24.016

Abstract

Abstract:

BACKGROUND: The effect of dimethyl sulfoxide cryoprotectants has been got a lot of verification in the low-temperature medical applications. But there is no literature addressing microdialysis detection of dimethyl sulfoxide cryoprotectants.
OBJECTIVE: To investigate the microdialysis relative recovery of different concentrations of dimethyl sulfoxide cryoprotectants used for limb reattachment.
METHODS: In vitro linear probe relative recovery of different concentrations of dimethyl sulfoxide (2%, 5%, 8%) was detected by retrodialysis gain and loss method. The correction in vivo experiment was done to estimate dimethyl sulfoxide relative recovery in rabbit amputated limbs.
RESULTS AND CONCLUSION: The relative recoveries of different concentrations of dimethyl sulfoxide (2%, 5%, 8%) were (49.49±3.56)%, (46.30±1.48)%, (52.66±2.54)% using retrodialysis gain method and (50.99±6.89)%, (43.86±1.35)%, (50.67±0.75)% using retrodialysis loss method. The average recoveries were (49.48±3.18)% and (48.51±4.03)%, respectively. There was no difference in the relative recovery of dimethyl sulfoxide detected using two methods. The change of dimethyl sulfoxide concentration could not influence the retrodialysis gain and loss method calibration results. The recovery was (15.45±4.8)% in vivo. These findings indicate that the microdialysis technology is suitable for dimethyl sulfoxide sampling in vivo that has no obvious influence on the relative recovery.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Dimethyl Sulfoxide, Microdialysis

CLC Number:

R318

An Zhuo-ling, Ding Kun, Liu Chun-sheng, Zhu Ze-xing, Zhou Jing-chao, Zhang Zhen, Zhang Shu-ming, Wang Dan . Dimethyl sulfoxide acts as a protective agent to perfuse rabbit amputated limbs: the relative recovery of local drug concentrations[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(24): 3855-3859.

Figures/Tables 2

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