Cardiac function and electrophysiological characteristics in myocardial infarction rats after tissue engineered cardiac patch transplantation

doi:10.3969/j.issn.2095-4344.2013.24.005

Abstract

Abstract:

BACKGROUND: The structure of tissue engineered cardiac muscle tissue is similar to heart tissue three-dimensional electric coupling network and muscle striations, and has myocardial tissue-like systolic functions, and it can be used to repair the injured cardiac tissue.
OBJECTIVE: To observe the cardiac functions and electrophysiological characteristics in myocardial infarction rats after myocardial cells/collagen complex transplantation.
METHODS: Adult Sprague-Dawley rats were randomly divided into three groups: sham surgery group, model group and transplantation group. Myocardial infarction models were developed in the model group and transplantation group, while sham surgery group was only subjected to thoracotomy with no ligation of coronary artery. Transplantation group was given myocardial cells/collagen complex transplantation, while sham surgery group and model group received no transplantation.
RESULTS AND CONCLUSION: (1) Left ventricular heart function: Compared with the sham surgery group, the left ventricular internal diameter at end-diastole and left ventricular internal diameter at end-systole were significantly increased (P < 0.01), while left ventricular ejection fraction and shortening fraction were decreased in the model group (P < 0.01). The above indexes were not changed in transplantation group (P > 0.01). (2) Left ventricular effective refractory period: Compared with the sham surgery group, effective refractory period at the infarct border zone was significantly shortened in the model group (P < 0.01); compared with the model group, effective refractory period at the infarct border zone was significantly prolonged in transplantation group (P < 0.01). (3) Connexin 43 immunofluorescence results: Connexin 43 expression was positive in sham surgery group, weakly positive in the model group and transplantation group. However, the positive expression in the transplantation group was higher than that in model group. Myocardial cells/collagen complex transplantation can improve ventricular myocardium systolic function and electrophysiological properties of myocardial infarction rats, through electric coupling network and contraction coupling in tissue and structure.

Key words: tissue construction, heart tissue construction, connexin 43, myocardial infarction, left ventricular cardiac function, tissue engineering, myocardial cells/collagen complex, electric coupling, effective refractory period, immunofluorescence, nfarct border zone, gap junction, provincial grants-supported paper

CLC Number:

R318

Adila•Azhati, Zhao Long, Wang Qian, Ma Yi-tong. Cardiac function and electrophysiological characteristics in myocardial infarction rats after tissue engineered cardiac patch transplantation[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(24): 4401-4408.

Figures/Tables 4

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