Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (43): 8067-8070.doi: 10.3969/j.issn.2095-4344.2012.43.019

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Effect of different pore sizes of hollow titanium alloy prosthesis’s wall on the growth of bone marrow stromal cells

Fan Yu-ping1, Huang Jing-xiang2, Cai Xu3   

  1. 1Department of Orthopedics, Ulanqab Central Hospital, Ulanqab 012000, Inner Mongolia Autonomous Region, China; 2Orthopedics Laboratory, 3Department of Orthopedics, General Hospital of Chinese PLA, Beijing 100853, China
  • Received:2012-02-02 Revised:2012-03-02 Online:2012-10-21 Published:2012-10-21
  • Contact: Cai Xu, Doctor, Chief physician, Department of Orthopedics, General Hospital of Chinese PLA, Beijing 100853, China
  • About author:Fan Yu-ping★, Master, Associate chief physician, Department of Orthopedics, Ulanqab Central Hospital, Ulanqab 012000, Inner Mongolia Autonomous Region, China Fanyuping1024@ 126.com

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Abstract:

BACKGROUND: The growth behavior of cells on a stent is influenced by the pore size and porosity of stents as well as many other factors.
OBJECTIVE: To observe the effect of different pore sizes of hollow titanium alloy prosthesis’s wall on the growth of bone marrow stromal cells (BMSCs).
METHODS: Hollow porous metal prostheses were divided into two groups by the pore size of the wall, which were φ1: 1 mm and φ2: 1.5 mm. The prosthesis was located in the BMSCs suspension for osteogenic induction. BMSCs without osteogenic induction served as controls. The cell morphology, growth and calcium nodules were observed under inverted phase contrast microscope.
RESULTS AND CONCLUSION: At 4 week, BMSCs were linked directly through the hole of the groupφ1, but not in the groupφ2. Calcium nodules were formed in the groupsφ1 andφ2. These indicate that BMSCs cultured in vitro can be linked directly in the proper pore size to prevent a potential gap between implants and the host bone. In addition, this spatial structure can provide sufficient space for the growth of regenerated bones.

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