Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (38): 7087-7091.doi: 10.3969/j.issn.2095-4344.2012.38.012

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Repairing peripheral nerve defects in rats by electrostatic spinning poly (lactic-co-glycolic acid)-silk fibroin-collagen nerve conduits

Li Sen1, Shi Xiao-yuan1, Lin Wei2, Fan Hong-song3, Chen Bao1, Wu Hui1, Hu Xu-dong1, Wang Guang-lin1   

  1. 1Department of Orthopedics, West China Hospital of Sichuan University, Chengdu 610041, Sichuan Province, China
    2West China Hospital of Gynecology, Obstetrics and Children, Sichuan University, Chengdu 610041, Sichuan Province, China
    3Engineering Research Center of Biomaterials, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2012-01-24 Revised:2012-02-06 Online:2012-09-16 Published:2012-09-16
  • Contact: Wang Guang-lin, Associate professor, Master’s supervisor, Department of Orthopedics, West China Hospital of Sichuan University, Chengdu 610041, Sichuan Province, China wglfrank@163.com
  • About author:Li Sen, Physician, Department of Orthopedics, West China Hospital of Sichuan University, Chengdu 610041, Sichuan Province, China lisensun@163.com

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Abstract:

BACKGROUND: The repair of peripheral nerve defects exhibits a clinical challenge. Because of the limitation of nerve autograft, it is a promising choice for artificial nerve grafts.
OBJECTIVE: To investigate the possibility of repairing sciatic nerve defects by electrostatic spinning poly (lactic-co-glycolic acid) (PLGA)-silk fibroin-collagen nano nerve conduits.
METHODS: Totally 36 female SD rats were underwent 10 mm sciatic nerve defects. The nerves of rats were grafted by reverse nerve autografts, electrostatic spinning PLGA-silk fibroin-collagen nerve conduits and PLGA nerve conduits alone. At week 12 postoperatively, nerve regeneration was studied by general observations, electrophysiological examination, microscopic observation, transmission electron microscopy observation and image analysis.
RESULTS AND CONCLUSION: The results showed that the nanofibers-based nerve conduits prepared by electrostatic spinning had a well-distributed porous structure and could simulate the structure of native extracellular matrix. These findings suggest that the electrostatic spinning PLGA-silk fibroin-collagen nerve conduits group is superior to PLGA nerve conduits group in sciatic nerve regeneration, regenerative nerve myelinization and nerve foundation reconstruction, but slightly worse than the nerve autograft group.

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