Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (2): 191-195.doi: 10.3969/j.issn.1673-8225.2011.02.001

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Construction of tissue-engineered bone using cell sheet technology

Chen Tao1, Wang Yan-hui1, Bu Ling-xue1, Jing Heng1, Gao Zhen-hua1, Tan Shuai1, Wang Ling-ling1, Li Ning-yi2   

  1. 1Deparment of Medical Image, Affiliated Hospital of Qingdao University Medical College, Qingdao   266003, Shandong Province, China
    2Department of Oral and Maxillofacial Surgery, Qingdao University Medical College, Qingdao   266003, Shandong Province, China
  • Received:2010-10-08 Revised:2011-01-10 Online:2011-01-08 Published:2011-01-08
  • Contact: Li Ning-yi, Doctor, Professor, Department of Oral and Maxillofacial Surgery, Qingdao University Medical College, Qingdao 266003, Shandong Province, China ningyili342@163.com
  • About author:Chen Tao★, Master, Associate professor, Deparment of Medical Image, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China Chentaojinxiang@ 163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30872896; the Natural Science Foundation of Shandong Province, No. Y2008C77

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Abstract:

BACKGROUND: Traditional bone tissue engineering faces shortcomings in harvesting or transferring cells. Cell sheet technology is a new skill for cell harvesting or transferring.
OBJECTIVE: To construct tissue-engineered bone using cell sheet technology and method of traditional bone tissue engineering.
METHODS: Canine bone marrow mesenchymal stem cells (BMSCs) were isolated with the method of density gradient centrifugation and cultured. BMSCs were induced to differentiate into osteoblasts and cultured in temperature-responsive culture dishes at 37 ℃, 5% CO2 saturated humidity. BMSCs cell sheet was prepared when temperature was changed to 20 ℃; demineralized bone matrix and platelet rich plasma (PRP) were prepared. The complex of DBM/PRP/BMSCs cell sheet/BMSCs was construsted and implanted under the left latissimus dorsi muscle. The condition of osteogenesis was observed at 4, 8 and 12 weeks after operation.
RESULTS AND CONCLUSION: When temperature dropped at 20 ℃, BMSCs detached automatically from the temperature-responsive culture dishes and formed an intact cell sheet. The osteogenesis of the DBM/PRP/BMSCs cell sheet/BMSCs group was better than that of the DBM/PRP/BMSCs group. Cell sheet technology combined with traditional bone tissue provides a new way for construction of functional tissue-engineered bone.

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