Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (2): 196-200.doi: 10.3969/j.issn.1673-8225.2010.02.002

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Expression of senescence-associated beta-galactosidase and c-Fos protein in osteoarthritic cartilage

Li Liang-jun1, Lei Guang-hua2, Tu Min2, Cheng Chao2, Chen Xia-guang2, Gao Shu-guang2, Zeng Kai-bin2   

  1. 1Department of Orthopaedics, Changsha Central Hospital, Changsha  410004, Hunan Province, China
    2Department of Orthopaedics, Xiangya Hospital, Institute of Orthopedics, Central South University, Changsha  410008, Hunan Province, China
  • Received:2010-07-24 Revised:2010-10-20 Online:2011-01-08 Published:2011-01-08
  • Contact: Lei Guang-hua,Department of Orthopaedics, Xiangya Hospital, Institute of Orthopedics, Central South University, Changsha 410008, Hunan Province, China lgh9640@sina.com
  • About author:Li Liang-jun★, Master, Physician, Department of Orthopaedics, Changsha Central Hospital, Changsha 410004, Hunan Province, China liliangjun1212@sina.com

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Abstract:

BACKGROUND: The expression of senescence-associated β-galactosidase (SA-β-gal) serves as a marker of cell senescence, while the expression of c-Fos in cartilage of osteoarthritis is rare.
OBJECTIVE: To study expression differences of SA-β-gal and c-Fos protein in normal and impaired cartilage of osteoarthritis.
METHODS: Ten normal cartilages and 26 cartilage of osteoarthritis were selected. On the basis of improved Mankin pathological score, 50 cartilage specimens to divide into normal, mild impaired, medium impaired and severe impaired groups. The expression of SA-β-gal and c-Fos protein in the cartilage specimens were detected by SABC method. The difference and correlation between the percentage of the positive chondrocytes and the different impaired cartilage were analyzed. 
RESULTS AND CONCLUSION: The cartilage degeneration aggravated with increasing of SA-beta-gal positive chondrocytes, suggesting that chondrocyte senescence has contacted with osteoarthritis. The expression of c-Fos protein shows no direct correlation with the severity of osteoarthritis and chondrocyte senescence. The findings demonstrated that chondrocyte senescence may be one of pathogenesis of osteoarthritis, which promotes compensate for cell division in the early and medium stage of osteoarthritis.

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