Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (1): 123-127.doi: 10.3969/j.issn.2095-4344.2016.01.022

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Molecular genetic analysis of genes from MNS, Duffy and Kell blood groups in the China Xinjiang Uygur population

Lin Guo-yue1, Du Xiao-lu1, Shan Jin-jing1, Zhang Ya-nan1, Zhang Yu-qiang1, Zhang Yuan-zhou2   

  1. 1Department of Blood Transfusion, 2Department of Medical Service, the 474th Hospital of PLA, Urumqi 830013, Xinjiang Uygur Autonomous Region, China
  • Received:2015-11-25 Online:2016-01-01 Published:2016-01-01
  • Contact: Zhang Yuan-zhou, Master, Attending physician, Department of Medical Service, the 474th Hospital of PLA, Urumqi 830013, Xinjiang Uygur Autonomous Region, China
  • About author:Lin Guo-yue, Master, Chief technician, Master’s supervisor, Department of Blood Transfusion, the 474th Hospital of PLA, Urumqi 830013, Xinjiang Uygur Autonomous Region, China
  • Supported by:

     the Medicine and Health Scientific Research Plan of Lanzhou Military Region, No. CLZ15JB26

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Abstract:

BACKGROUND: Screening of fare blood types has been successively implemented and completed in Europe, America and Japan, but there is a large gap in China. Previous studies have mainly focused on the southern Han populations, and little is reported on PCR-SSP systematic analysis of gene frequencies of rare blood groups in Xinjiang Uygur populations.
OBJECTIVE: To investigate the gene frequency distribution of RBC MNS, Duffy, Kell, Dombrock, Diego, Kidd, Scianna, Colton and Lutheran blood groups from Xinjiang Uygur populations, thereby providing a strategic support for human population genetics and clinical blood deployment.
METHODS: PCR-SSP method was used to make genotyping and statistical analysis in 158 Xinjiang Uygur persons from nine rare blood groups.
RESULTS AND CONCLUSION: Gene frequencies of these nine rare blood groups were M=0.579 1, N=0.420 9, S=0.174 3, s=0.800 9, Fya=0.699 4, Fyb=0.300 6, K1=0.015 8, K2=0.984 2, Doa=0.234 2, Dob=0.765 8, Dia=0.047 4, Dib=0.952 6, JKa=0.541 2, JKb=0.452 6, Sc1=1.000, Sc2=0, Coa=0.994, Cob=0.005 9, Lua=0, Lub=1.000, Aua=0.810 2, Aub=0.189 9. Results from chi-square test showed that the observed value and expected value of genotypes were in line with the law of Hardy-Weinberg equilibrium (P > 0.05), and in the MNS blood group of Xinjiang Uygur population, it was rarely found that S-s- frequency was 0.025 3 in four cases and Jka-b- frequency was 0.006 3 in one case. This study demonstrates that the frequency distribution of MNS, Duffy, Dombrock and Diego blood groups in the Xinjiang Uygur population, with its own unique frequency distribution characteristics, is different from that in other ethnic populations; the gene distribution of Kell, Kidd and Colton blood groups shows either similarity or difference between the Xinjiang Uygur population and reported Tibet and Han populations; Scianna and Lutheran blood groups show a monomorphic distribution in the Xinjiang Uygur population, which is similar to that in the Tibet and Han populations. These findings provide the basic data for exploring the origin and evolution, ethnic hematology and construction of rare blood database of the Xinjiang Uygur population.