Expression of inflammatory substances in the synovial fluid of patients with knee osteoarthritis

doi:10.3969/j.issn.2095-4344.2015.15.008

Abstract

Abstract:

BACKGROUND: The main change of knee osteoarthritis is cartilaginous degeneration and secondary bone hyperplasia. The mechanism of these pathological changes is unclear, but some experiments have proved that the onset of knees osteoarthritis is closely related to inflammatory substances.
OBJECTIVE: To discuss the role of inflammatory substances in the pathogenesis of knee osteoarthritis.
METHODS: According to diagnostic criteria set by the American College of Rheumatology, we selected 60 knee osteoarthritis patients as experimental group, and another 60 patients undergoing the operative treatment of traumatic amputation or meniscus injury (except knee injury) as control group. After extracting synovial fluid samples from two groups, ELISA assay method was used to detect the levels of interleukin-1β, interleukin-6, interleukin-8, interleukin-10, tumor necrosis factor α, basic fibroblast growth factor, and osteopontin. The nitric oxide level in the synovial fluid was detected with nitric oxide detection kit. The content of lipid peroxidase was detected using TBA fluorescence method.
RESULTS AND CONCLUSION: We found higher levels of interleukin-1β, interleukin-6, interleukin-8, interleukin-10, tumor necrosis factor α, basic fibroblast growth factor, osteopontin, nitric oxide, and lipid peroxidase in knee osteoarthritis patients, and these levels were positively correlated with knee osteoarthritis. These inflammatory substances are really involved in the inflammation process of knees osteoarthritis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: Cytokines, Knee, Osteoarthritis

CLC Number:

R318

Ren Hai-liang, Ma Jian-xiong, Ma Xin-long. Expression of inflammatory substances in the synovial fluid of patients with knee osteoarthritis[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(15): 2336-2340.

Figures/Tables 1

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