Xenogeneic bone properties prepared by calcination and freeze-dried methods

doi:10.3969/j.issn.2095-4344.2013.51.002

Abstract

Abstract:

BACKGROUND: Xenogeneic bone structure and biological characteristics are similar to human bone tissue, and the xenogeneic bone has a decreased antigenicity after physicochemical treatment, with a natural porous structure and rich source, and can be kept for a long time, which is considered to be an effective way to solve the shortage of the autogenous bone and allograft bone.
OBJECTIVE: To compare the physical and chemical properties of xenogenic bone materials prepared by two different methods.
METHODS: Sheep cancellous bone treated with chemical method was placed into the muffle furnace at 1 000 ℃ for 2 hours to prepare calcined bone. Another cancellous bone was placed into an 80 ℃ refrigerator for 4 weeks and then placed into a vacuum instrument to prepare freeze-dried bone. Cancellous bone rinsed with ultra-pure water served as controls.
RESULTS AND CONCLUSION: Three groups of samples retained three-dimensional porous structure which similar with human bone tissue under microscopic observation. The framework was still intact, with a small pore of 55-650 μm and high porosity of 65%-80%. For the calcined bone, the toughness was decreased and the brittleness increased significantly, but the freeze-dried bone had a little changes in the mechanical properties. Through diffraction analysis, hydroxyapatite was the main composition of the three groups. However, there was a small amount of β-tricalcium phosphate in the calcined bone. Spectrum analysis confirmed that calcium and phosphorus content in these three groups were all close to the human body. The results suggest the cancellous bone treated with these two methods is similar to human bone structure, and the major elements are close to the body. In addition, the cancellous bone after processing has enough small pore and higher porosity. However, calcination process has a more influence on the mechanical property of scaffold materials, and the freeze-dried bone has a little change but the antigen cannot be completely removed that can reach the basic requirements.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

CLC Number:

R318

Yang Ze-hui, He Hui-yu, Cui Jie, Wang Xiao-zhi, Zhou Zheng. Xenogeneic bone properties prepared by calcination and freeze-dried methods[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(51): 8788-8794.

Figures/Tables 7

References

[1]Louis-Ugbo J, Murakami H, Kim HS, et al. Evidence of osteoinduction by Grafton demineralized bone matrix in nonhuman primate spinal fusion. Spine (Phila Pa 1976). 2004;29(4):360-366; discussion Z1.

[2]Athanasiou VT, Papachristou DJ, Panagopoulos A, et al. Histological comparison of autograft, allograft-DBM, xenograft, and synthetic grafts in a trabecular bone defect: an experimental study in rabbits. Med Sci Monit. 2010;16(1): BR24-31.

[3]Develioglu H, Unver Saraydin S, Kartal U. The bone-healing effect of a xenograft in a rat calvarial defect model. Dent Mater J. 2009;28(4):396-400.

[4]Park SA, Shin JW, Yang YI, et al. In vitro study of osteogenic differentiation of bone marrow stromal cells on heat-treated porcine trabecular bone blocks. Biomaterials. 2004;25(3): 527-535.

[5]Mastrogiacomo M, Muraglia A, Komlev V, et al. Tissue engineering of bone: search for a better scaffold. Orthod Craniofac Res. 2005;8(4):277-284.

[6]Erdemli O, Captug O, Bilgili H, et al. In vitro and in vivo evaluation of the effects of demineralized bone matrix or calcium sulfate addition to polycaprolactone-bioglass composites. J Mater Sci Mater Med. 2010;21(1):295-308.

[7]Jähn K, Braunstein V, Furlong PI, et al. A rapid method for the generation of uniform acellular bone explants: a technical note. J Orthop Surg Res. 2010;5:32.

[8]张富强.快速成型在生物医学工程的应用[M].北京:人民军医出版社,2009:142-143.

[9]邢辉,陈晓明,张宏泉.骨组织工程支架材料[J].生物骨科材料与临床研究,2004,1(5):35-39.

[10]Mastrogiacomo M, Muraglia A, Komlev V, et al. Tissue engineering of bone: search for a better scaffold. Orthod Craniofac Res. 2005;8(4):277-284.

[11]郑磊,王前,裴国献. 骨组织工程中细胞外基质材料的选择[J].中华外科杂志,2000,38(10):745-748.

[12]Simon CG Jr, Khatri CA, Wight SA, et al. Preliminary report on the biocompatibility of a moldable, resorbable, composite bone graft consisting of calcium phosphate cement and poly(lactide-co-glycolide) microspheres. J Orthop Res. 2002; 20(3):473-482.

[13]许永华,施新猷,胡蕴玉,等.胶原-煅烧骨支架与成骨细胞相容性实验研究[J].中国临床解剖学杂志,2000,18(4):358-359.

[14]王金成,李志洲,陈伟,等,骨库骨的生物力学特性比较研究[J].骨与关节损伤杂志,2001,16(1):35-36.

[15]杨春蓉.骨组织工程支架研究现状及面临的问题[J].中国组织工程研究与临床康复,2009,13(8):1529-1532.

[16]邓红文,刘耀中.骨生物学前沿[M].北京:高等教育出版社.2006: 110-131.

[17]刘雷,李起鸿,唐康来,等.异种脱蛋白组织工程骨支架材料的制备及理化特性研究[J].第三军医大学学报,2007,29(1):12-14.

[18]Zhao F, Yin Y, Lu WW, et al. Preparation and histological evaluation of biomimetic three-dimensional hydroxyapatite/ chitosan-gelatin network composite scaffolds. Biomaterials. 2002;23(15):3227-3234.

[19]Kurashina K, Kurita H, Wu Q, et al. Ectopic osteogenesis with biphasic ceramics of hydroxyapatite and tricalcium phosphate in rabbits. Biomaterials. 2002;23(2):407-412.

[20]Ramay HR, Zhang M. Biphasic calcium phosphate nanocomposite porous scaffolds for load-bearing bone tissue engineering. Biomaterials. 2004;25(21):5171-5180.