Changes of related factors on the pressure side under different appliance orthodontic during periodontal bone remodeling

doi:10.3969/j.issn.2095-4344.2013.20.009

Abstract

Abstract:

BACKGROUND: Recent studies have shown that receptor activator of nuclear factor-κB ligand is closely related to osteoclast differentiation, formation and function in bone remodeling during orthodontic tooth movement.
OBJECTIVE: To observe the changes in receptor activator of nuclear factor-κB ligand expression in the pressure side during periodontal tissue remodeling under different appliance orthodontics, and to explore the effect of different orthodontic appliances.
METHODS: Sixty-four healthy rabbits were divided into four groups: control group, MBT appliance group, Begg appliance group, Damon Ⅲ appliance group, 16 rabbits in each group. In the MBT appliance group, Begg appliance group and Damon Ⅲ appliance group, corresponding appliances were used to correct the maxillary incisors and first molars respectively, and the mesial movement traction force was 80 g; the control group did not receive correction. Four rabbits were selected from each group for testing at 3, 7, 14 and 21 days after correction.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining showed that the periodontal ligament cavity in the pressure side was narrow and the edge of the alveolar bone exhibited resorption pits. Tartrate-resistant acid phosphatase staining showed bone remodeling was active and the number of osteoclasts reached peak at 7 days after correction. Real-time quantitative PCR showed that the mRNA expression of receptor activator of nuclear factor-κB ligand on the pressure side of the alveolar bone tissue was significantly increased after force loading, and reached peak at 7 days after forcing, then gradually decreased. At 7 days after forcing, the number of osteoclasts and the mRNA expression of receptor activator of nuclear factor-κB ligand in the Damon Ⅲ appliance group were higher than those in the MBT appliance group and Begg appliance group (P < 0.05). In bone remodeling process, mRNA expression of receptor activator of nuclear factor-κB ligand was positively correlated with the number of osteoclasts, and the effect of Damon Ⅲ appliance was better than MBT and Begg appliances.

Key words: tissue construction, oral tissue construction, Damon Ⅲ appliance, MBT appliance, Begg appliance, orthodontics, periodontal tissues, receptor activator of nuclear factor-κB ligand, pressure, osteoclasts

CLC Number:

R318

Ma Yong-ping, Ge Chang-qing, Gao Lin-qing. Changes of related factors on the pressure side under different appliance orthodontic during periodontal bone remodeling [J]. Chinese Journal of Tissue Engineering Research, 2013, 17(20): 3671-3678.

Figures/Tables 5

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