Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (50): 9479-9484.doi: 10.3969/j.issn.2095-4344.2012.50.030

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Immune escape and biomechanics in the construction of tissue engineered cartilage tissue

Li Bin1, Wen Yu2, Yi Xian-hong2, Pan Jun3   

  • Online:2012-12-09 Published:2012-12-09

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Abstract:

BACKGROUND: Mechanical stress plays a very important role in cartilage tissue engineering, but there are fewer studies on stress induced allogeneic seed cell differentiation and the mechanism of stress to promote differentiation of allogeneic stem cells into chondrocytes is still unclear.

OBJECTIVE: To explore the feasibility of reducing immunogenicity of stem cells to apply allogeneic seed cells in the construction of tissue-engineered cartilage in clinic.

METHODS: A computer-based search of PubMed (2000-01/2012-01) was performed using the keywords of “engineered articular cartilage tissue, cartilage immune, articular cartilage, biomechanical”. The language was limited to English. Totally 510 articles were retrieved, and finally 33 articles were included in result analysis.

RESULTS AND CONCLUSION: Stem cells with multilineage differentiation potential differentiate into chondrocytes. Mechanical load effects on chondrocytes and mechanical signal transduction mechanism have been explored to carry out a large-scale proliferation of chondrocytes using bioreactor. Gene targeting technology enables the recombination of chromosomal DNA from stem cells cultured in vitro and exogenous homologous sequence to modify β2-microglobulin gene, reduce the immunogenicity of stem cells, and provide low-immunogenicity seed cells for cartilage tissue engineering.