Abstract:

BACKGROUND: Platelet rich plasma can reactivate the bone tissue, while some basic problems are still unknown such as the ideal platelet concentration, the important elements and relevant mechanisms.
OBJECTIVE: To investigate the correlation of washed platelet between mouse osteoblast MC3T3-E1 cell proliferation and prostaglandin E2 production.
METHODS: Washed platelet was obtained from young healthy male volunteer and acted on MC3T3-E1 after repeatedly extracted from liquid nitrogen frozen. Cell quantification kit and prostaglandin E2 assay kit were applied to detect cell proliferation and PGE2 production. Reverse transcription-PCR was used to detect the cyclooxygenase-2 mRNA expression.
RESULTS AND CONCLUSION: After 5% washed platelet acted on MC3T3-E1 cells for 1 hour, cyclooxygenase-2 mRNA expression and prostaglandin E2 production were promoted. Cyclooxygenase-2 mRNA expression reached a peak value at 3 hours; while prostaglandin E2 production reached a peak value at 6 hours (40.5 μg/L). The promotive effect of washed platelet on the proliferation of MC3T3-E1 cells was decreased with the concentration increased. Washed platelet at 15% inhibited the MC3T3-El cell proliferation. However, prostaglandin E2 production of MC3T3-E1 cells was promoted with the washed platelet concentration increased. Indomethacin significantly inhibited the effect of 50 moL/L washed platelet on MC3T3-E1 cell proliferation and prostaglandin E2 production. However, tumor necrosis factor-2 (100 μg/L) could significantly promote the effect of washed platelet on prostaglandin E2 production of MC3T3-E1 cells. Moreover, SB431542 (15 μmol/L) significantly inhibited the promotive effect of 5% washed platelet on MC3T3-E1 cell proliferation and prostaglandin E2 production. These results suggest that washed platelet promoting MC3T3-El proliferation is closely related to prostaglandin E2 production