Abstract:

BACKGROUND: To establish an experimental rat model of periodontitis is one of the basic methods to study periodontitis. However, the commonly used methods have many drawbacks and need further improvement.
OBJECTIVE: To investigate a new method that can establish an animal model of periodontitis similar to the human body.
METHODS: Twenty rats (8 weeks of age) were randomly divided into a control group and experimental group. In the experimental group, the buccolingual gingival of the first maxillary molar was sharply separated, dental calculi derived from chronic periodontitis patients were dried, crushed and adhered to the tooth neck; after that, 10 μL salivary of chronic periodontitis patients was dropped into the gingival twice every day. The contry group had no treatment. The amimals were all provided with high glucose food and drink.
RESULTS AND CONCLUSION: After 14 days of modeling, the general biological characteristics, pathological, and X-ray changes of the periodontal tissue of the model animals were dramatically in accord with typical human periodontitis. The rat model of periodontitis established by adhering dental calculus and mixed bacterial infection can simulate human periodontitis. It is a simple and effective method.