Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (37): 6863-6866.doi: 10.3969/j.issn.2095-4344.2012.37.005

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Establishment of a neointimal model of pulmonary hypertension induced by monocrotaline injury plus pneumonectomy in rats

Liang Fu-qiu1, Lin Qun2, Lin Cai-zhu2, Lei Li-hua3, Yang Qing2, Lin Xian-zhong2, Cai Hong-da2   

  1. 1Fujian Medical University, Fuzhou 350004, Fujian Province, China
    2Department of Anesthesiology, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, Fujian Province, China
    3Department of Anesthesiology, Teaching Hospital of Fujian Medical University, Fujian Provincial Hospital, Fuzhou 350014, Fujian Province, China
  • Received:2012-01-08 Revised:2012-02-04 Online:2012-09-09 Published:2012-09-09
  • Contact: Lin Qun, Associate chief physician, Associate professor, Master’s supervisor, Department of Anesthesiology, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, Fujian Province, China linqun007@163.com
  • About author:Liang Fu-qiu★, Studying for master’s degree, Physician, Fujian Medical University, Fuzhou 350004, Fujian Province, China 409273133@qq.com

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Abstract:

BACKGROUND: There is currently a lack of simple, practical, workable neointimal models of pulmonary hypertension in rats.
OBJECTIVE: To establish a neointimal model of pulmonary hypertension by monocrtaline injury plus pneumonectomy in rats.
METHODS: Forty male SD rats were randomly divided into two groups: monocrtaline+pneumonectomy group (experimental group) was subjected to left pneumonectomy followed by monocrotaline injected 2 weeks later (n=26); Control group only underwent sham operation (n=14). Pulmonary artery pressure, ventricular weight, and pulmonary artery morphology were evaluated at 5 weeks after operation in each group. Factor VIII-positive capillaries that were less than 100 μm in diameter were counted under microscopy for 10 randomly selected fields per specimen. The capillary density was determined as the average number of factor VIII-positive capillaries per 1 mm2.
RESULTS AND CONCLUSION: Four weeks after operation, 85% rats survived (22/26) in the experimental group, and 100% in the control group (14/14). The experimental group had significantly higher mean pulmonary artery pressures and ratio of right ventricular and left ventricular plus septal weights, as well as percent wall thickness of muscular pulmonary arteries ranging from 50 to 100 μm diameter and from 100 to 150 μm diameter were significantly increased as compared with the control group (P < 0.01). Pulmonary capillary density was less in the experimental group than in the control group (P < 0.01). Historic examination showed significant medial hypertrophy of muscularized pulmonary arteries and muscularization of intra-acinar pulmonary arteries and intimal thickening developed in right lung intracinar pulmonary arteries in the experimental group. However, there was no evidence of pulmonary vascular remodeling in the control group. Hemodynamic pulmonary hypertension and neointimal lesions could be quickly induced at 5 weeks after pneumonectomy plus monocrotaline injury. This method was easily performed and mortality of rats was relatively low.

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