Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (37): 6857-6862.doi: 10.3969/j.issn.2095-4344.2012.37.004

Previous Articles     Next Articles

Gene-expression profiles about myocardial ischemia injury miniature pig’s growth function and intervention effects of needle-embedding therapy at Neiguan

Yang Xiao-fang1, Cui Jin1, Liu Xiao-yu2, Zhang Xiao-shan1, Feng Lin1, Wang Xing-gui1, Qian Ning3   

  1. 1Department of Acupuncture, Moxibustion and Tuina, 3Research Institute of Zoology, Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China; 2Department of Traditional Chinese Medicine, the Tenth People’s Hospital of Tongji University, Shanghai 200072, China
  • Received:2012-06-09 Revised:2012-07-15 Online:2012-09-09 Published:2012-09-09
  • Contact: Cui Jin, Doctor, Professor, Department of Acupuncture, Moxibustion and Tuina, Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China
  • About author:Yang Xiao-fang☆, Doctor, Associate professor, Master’s supervisor, Department of Acupuncture, Moxibustion and Tuina, Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China yangxiaofang210@163.com

PDF

535

Abstract:

BACKGROUND: Acupuncture at Neiguan (PC6) can up-regulate relative expressions of vascular growth factors. Nonetheless, there are plenty of growth factors related to angiogenesis, and researches have limitations if just focusing on a part of these factors.
OBJECTIVE: To observe characteristics on gene-expression profiles about myocardial ischemia injury miniature pig’s growth function and intervention effects of needle-embedding therapy at Neiguan.
METHODS: Thirty-two miniature pigs were randomized into four groups. Afterwards, ligation of the left anterior descending coronary artery was performed to establish myocardial ischemia models. Meanwhile, pigs in sham operation group only underwent threading without ligation, while needle-embedding therapy at Neiguan and Geshu (BL17) was conducted in Neiguan group and Geshu group, respectively. Then, ischemic myocardial tissue was extracted for experiments after 7 days needle-embedding therapy.
RESULTS AND CONCLUSION: A total of 96 genes were detected that were closely related to the function of angiogenesis by using Q Series Angiogenesis’ functional genomics expression profiles. Compared with the model group, there were 17 significantly up-regulated genes and 3 down-regulated genes in the Neiguan group; and 14 significantly up-regulated genes and 2 down-regulated genes in the Geshu group. As to classifications of gene function, differentially expressed genes had their own features when comparing differences expressions among Neiguan group, Geshu group and model group. Nevertheless, distinctive promoting factor and inhibitor, platelet derived growth factor and receptor, fibroblast growth factor and receptor, growth factor and receptor, other cell growth factors, cytokine and chemokines active element, matrix metalloproteinase and inhibitor, as well as other relative genes were shared between groups; among them, angiogenesis factors and receptors were the most. It indicates that intervention for myocardial ischemia and needle-embedding therapy are related to angiogenesis factors, but the action mechanism of needle-embedding therapy at Neiguan is different from that at Geshu.

CLC Number: