Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (34): 6326-6330.doi: 10.3969/j.issn.2095-4344.2012.34.012

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Tumor-targeted nanoparticles derived from pullulan acetate conjugate: Preparation, stability and release in vitro

Tang Hong-bo1, 2, Chen Hong-li3, Zhou Zhi-min2, Zhang Tong2, Liu Ling-rong2, Zhang Qi-qing2, 4   

  1. 1Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100007, China;
    2Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300192, China;
    3The Key Laboratory of Hereditary Disease and Molecular Target Drug Therapy, Department of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, Henan Province, China;
    4Research Center of Biomedical Engineering, Medical College of Xiamen University, Xiamen 361005, Fujian Province, China
  • Received:2012-02-05 Revised:2012-06-28 Online:2012-08-19 Published:2012-08-19
  • Contact: Zhang Qi-qing, Doctor, Professor, Doctoral supervisor, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300192, China; Research Center of Biomedical Engineering, Medical College of Xiamen University, Xiamen 361005, Fujian Province, China zhangqiq@126.com
  • About author:Tang Hong-bo☆, Doctor, Pharmacist in charge, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100007, China; Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300192, China tanghongbo77@ gmail.com

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Abstract:

BACKGROUND: Pullulan due to its many unique characteristics have received more and more attention in the field of drug delivery systems. But, the tumor targeted nano-drug carriers based on pullulan needed to be further studied and developed.
OBJECTIVE: To observe the stability and drug release in vitro of nano-drug carriers and to preliminarily evaluate the potential of folate conjugated pullulan acetate (FPA) as a nano-drug carrier.
METHODS: Folate was coupled to pullulan acetate (PA). FPA nanoparticles (FPAN) and epirubicin-loaded FPA nanoparticles (FPA/EPI) were prepared by dialysis method. The storage stability of FPAN and FPA/EPI was observed by storage method, and the in-vitro release characteristics were studied by dialysis bag method.
RESULTS AND CONCLUSION: FPAN and FPA/EPI had the nearly spherical shape with a size range of (204.2±10.9) nm and (273.4±11.0) nm, respectively, and they had low ζ potentials both in water and in 10% fetal bovine serum. FPAN maintained stable for at least 1 year. The drug encapsulated in FPAN was released more quickly in pH 5.0 PBS than in pH 7.4. It is concluded that the FPA nano-drug carrier is easy to prepare and has good stability. FPA and FPA/EPI nanoparticles have the potential to be new tumor-targeted nano-drug delivery systems.

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