Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (28): 5287-5290.doi: 10.3969/j.issn.2095-4344.2012.28.032

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Expression and application of calreticulin in the construction of cardiac histiocyte oxidative stress and ischemic injury

Li Lei-yong1, Tian Yue-feng2, Yuan Ye2, Wang Jun2, Zhang Bin-ren2, Wang Xu2   

  1. 1Laboratory Department, Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China;
    2School of Acupuncture and Moxibustion, Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, Shanxi Province, China
  • Received:2011-09-21 Revised:2011-10-24 Online:2012-07-08 Published:2012-07-08
  • Contact: Tian Yue-feng, Professor, School of Acupuncture and Moxibustion, Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, Shanxi Province, China tyfsx@163.com
  • About author:Li Lei-yong, Associate chief technician, Laboratory Department, Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China

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Abstract:

BACKGROUND: Calreticulin regulates many physiological and pathophysiological processes in vivo, such as cell apoptosis, stress and cardiovascular inflammatory response.
OBJECTIVE: To analyze the expression and application of calreticulin in ischemia-reperfusion injury.
METHODS: An online search of PubMed and CNKI databases was performed for relevant articles published from 1996 to 2010. Articles on the structure and function of calreticulin, the role of calreticulin in autoimmunization and cardiac development, the expression change of calreticulin in the ischemia-reperfusion process were included. On that basis, the expression changes of calreticulin in oxidative stress and ischemia-reperfusion injury were observed by experiment.
RESULTS AND CONCLUSION: Calreticulin plays an important role in the genesis, development and pathological changes of hearts by assisting protein folding correctly, maintaining the intracellular Ca2+ homeostasis as well as involving in the regulation of apoptosis, adhesion and the expression of steroid sensitive genes. Calreticulin expression in myocardial ischemia-reperfusion injury was detected by enzyme-linked immunosorbent assay, immunohistochemistry and RT-PCR using myocardial ischemia-reperfusion injury model in rats. The results indicate that calreticulin expression is increased after myocardial ischemia-reperfusion injury, and then the apoptosis signal pathway in endoplasmic reticulum is activated, leading to cell injury and aggravate the myocardial injury.

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