Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (30): 5596-5599.doi: 10.3969/j.issn.1673-8225.2011.30.021

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Micro CT observation on femoral metaphysis after spinal cord injury and immobilization in rats

Yu Wen-chao, Liu Yan, Yuan Wen, Guo Yong-fei, Cao Zhen-yu, Zou Han-lin   

  1. Department of Orthopedics, Changzheng Hospital, Second Military Medical University of Chinese PLA, Shanghai  200003, China
  • Received:2011-03-30 Revised:2011-05-03 Online:2011-07-23 Published:2011-07-23
  • Contact: Liu Yan, Associate professor, Associate chief physician, Department of Orthopedics, Changzheng Hospital, Second Military Medical University of Chinese PLA, Shanghai 200003, China liuyan318@yahoo.cn
  • About author:Yu Wen-chao★, Studying for master’s degree, Department of Orthopedics, Changzheng Hospital, Second Military Medical University of Chinese PLA, Shanghai 200003, China yuwenchao0117@163.com
  • Supported by:

    Science Research Plan of Shanghai Science and Technology Committee, No. 08411965800*

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Abstract:

BACKGROUND: Osteoporosis results from spine cord injury (SCI), due to a great deal of bone loss.
OBJECTIVE: To make an observation and comparison on bone density and bone microstructure at the distal femora changes after both SCI in early stage and osteoporosis due to immobilization.
METHODS: The SD rats were randomly divided into three groups. In the control group, T10 vertebrae plate resection was performed to rats without injuring dura mate of spinal cord and spinal cord. T10 vertebrae plate resection was also performed to rats in the SCI group with spinal cord injury by Allen’s method. Rats in the immobilization group were immobilized by limb-tail fixation at bilateral hindlimbs. The femoral metaphysis, ulna and radius were chosen for detection of bone density individuals and Micro CT scanning was performed to the other femoral metaphysis. 
RESULTS AND CONCLUSION: Compared with the control group, bone density, bone mineral content, bone volume/ total volume (BV/TV) , trabecular thickness (Tb.Th), cortical bone area (Ct.Ar), cortical thickness (Ct.Th), trabecular number (Tb.N) in the distal femur of rats in the SCI group and immobilization group deceased dramatically respectively (P < 0.05). Structure model index (SMI), bone surface/ total volume (BS/TV), trabecular separation/spacing (Tb.Sp) in the distal femur of the SCI group was higher than that of the control group (P < 0.05 ). No bone density difference of significance was found in the elbow-bone and radius between the three groups. Above all, both SCI and immobilization can result in osteoporosis. SCI have resulted in significantly changes in the bone microstructure in early stage and compared with the immobilization group, the degree of changes was more seriously.

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