Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (29): 5389-5392.doi: 10.3969/j.issn.1673-8225.2011.29.017

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Chitosan effect on femoral artery anastomosis patency rate in rats

Wang Ya-feng1, Yuan Zheng-jiang1, Zhao Ai-juan1, Zhang Xin-ying1, Liang Hao2   

  1. 1Department of Microsurgery, 2Department of Urology, the First Affiliated Hospital of Henan University of Science and Technology, Luoyang  471003, Henan Province, China
  • Received:2010-12-09 Revised:2011-02-23 Online:2011-07-16 Published:2011-07-16
  • Contact: Yuan Zheng-jiang, Chief physician, Department of Microsurgery, the First Affiliated Hospital of Henan University of Science and Technology, Luoyang 471003, Henan Province, China luoyangwangyafeng@163.com
  • About author:Wang Ya-feng★, Studying for master’s degree, Physician, Department of Microsurgery, the First Affiliated Hospital of Henan University of Science and Technology, Luoyang 471003, Henan Province, China

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Abstract:

BACKGROUND: Chitosan can effectively prevent postoperative tissue adhesion. But in amputation, whether replantation surgery has effects on vascular anastomosis is less reported.
OBJECTIVE: To study the medical effects of chitosan on adult rat femoral artery anastomosis patency rate. 
METHODS: Bilateral transection of the femoral artery was performed in SD rats, left side as the experimental group, and contralateral (right) as the control group. Anatomosis of bilateral femoral artery was performed under a microscope, all the immediate arterial anastomosis patency rate was 100%, and the same person operated. One drop of chitosan was covered surrounding the anatomosis site in the experiment group. After 7, 14, 21, 28 days, anastomotic patency was observed as well as anastomotic diameter, with or without infection around the stoma, adhesions around the anastomosis, anastomotic coverage of endothelial cells (biopsy).
RESULTS AND CONCLUSION: Preoperative and postoperative anastomotic diameter had no significant changes in the experimental group and control group without obvious infection around the stoma. The adhesion was slighter in the experimental group than in the control group. The overall anastomotic patency rate was 96% in the experimental group, and 89% in the control group. At various time periods after operation, endothelial cell coverage had no difference between the two groups. Chitosan does not affect the rat femoral artery anastomosis overall patency rate.

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