Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (29): 5384-5388.doi: 10.3969/j.issn.1673-8225.2011.29.016

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Biocompatibility of nano-bionic scaffolds

Yuan Shi-min1, Liu Ning2, Qiu Qing-zhong1   

  1. 1Department of Orthopedics, Guangdong Hospital of Integrated Chinese Medicine and Western Medicine, Foshan  528200, Guangdong Province, China
    2Department of Orthopedics, First Affiliated Hospital of Jinan University Medical School, Guangzhou  511632, Guangdong Province, China
  • Received:2011-03-16 Revised:2011-05-17 Online:2011-07-16 Published:2011-07-16
  • About author:Yuan Shi-min★, Master, Attending physician, Department of Orthopedics, Guangdong Hospital of Integrated Chinese Medicine and Western Medicine, Foshan 528200, Guangdong Province, China

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Abstract:

BACKGROUND: Nano-materials to build tissue engineered bone with bioactivity can simulate the structure of the extracellular matrix that is conducive to cell adhesion and growth.
OBJECTIVE: To investigate the in vitro histocompatibility of nano chitosan-sodium\collagen (nano-CS\COL) scaffolds.
METHODS: Bone marrow mesenchymal stem cells (BMSCs) were isolated from SD rats and cultured, and then were identified by morphology observations with the optical microscope and the exterior antigen through the flow cytometry method. Nano-CS\COL biomimetic scaffolds were prepared through the polyelectrolyte complex coacervation, then were co-cultured with BMSCs. The cellular compatibility was detected through the adherent rate, growth curve, cell livingness and cell cycle, dyeingⅠcollagen and morphology observations by the scanning electron microscope.
RESULTS AND CONCLUSION: The BMSCs could be obtained and cultured, express CD29, CD44, CD106, not CD34, CD45. The diameter of the nano-CS-COL scaffold was 150 μm with good histocompatibility to BMSCs. The BMSCs can be obtained and cultured stably for a long term in vitro, which are the ideal choice for tissue engineering. Nano-CS/COL scaffolds can be used as tissue engineering biomaterials for their good histocompatibility.

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