Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (29): 5375-5378.doi: 10.3969/j.issn.1673-8225.2011.29.014

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Biocompatibility of human umbilical cord blood derived mesenchymal stem cells and beta-tricalcium phosphate

Wang Qi, Liu Xian-min, Zu Qi-ming, Liu Song-bo, Cao Yan, Xiang Liang-bi   

  1. Department of Orthopaedics, General Hospital of Shenyang Military Area Command of Chinese PLA, Shenyang  110016, Liaoning Province, China
  • Received:2011-01-15 Revised:2011-02-09 Online:2011-07-16 Published:2011-07-16
  • Contact: Liu Xian-min, Chief physician, Department of Orthopaedics, General Hospital of Shenyang Military Area Command of Chinese PLA, Shenyang 110016, Liaoning Province, China Correspondence to: Xiang Liang-bi, Chief physician, Department of Orthopaedics, General Hospital of Shenyang Military Area Command of Chinese PLA, Shenyang 110016, Liaoning Province, China
  • About author:Wang Qi☆, Doctor, Attending physician, Department of Orthopaedics, General Hospital of Shenyang Military Area Command of Chinese PLA, Shenyang 110016, Liaoning Province, China ven15@126.com
  • Supported by:

    the Science and Technology Project of Liaoning Province, No. 2010225036-12*; the Key Topic of General Hospital of Shenyang Military Area Command of Chinese PLA, No. 08Y-Z18*

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Abstract:

BACKGROUND: Experiments in vivo has showed that β-tricalcium phosphate (β-TCP) porous ceramic is an ideal bone tissue engineering scaffold material, but it cannot reflect growth, proliferation, and phenotypic changes of cells well, due to implantation of experiments in vivo was affected by various factors.
OBJECTIVE: To investigate the biocompatibility of human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) in vitro and β-TCP porous ceramic.
METHODS: The 6th passage hUCB-MSCs suspension were dripped into β-TCP to undergo combination.The compound of stem cells and scaffold materials was transferred into the culture medium comprising α-minimum essential medium (α-MEM) supplemented with 10% fetal bovine serum (FBS). The surface of materials and internal growth status of hUCB-MSCs was observed under scanning electron microscope (SEM) at 4, 8, and 12 days. MTT assay was used to draw curve of cell growth and the DNA and protein content were determined.
RESULTS AND CONCLUSION: HUCB-MSCs can attach in the surface and internal pores of β-TCP scaffold materials after hUCB-MSCs combined with β-TCP, and its growth is good, which DNA replication and protein synthesis function were not affected by β-TCP. It suggested that hUCB-MSCs and β-TCP scaffold materials have good biocompatibility, and which can be used as seed cells and scaffold materials for tissue engineering bone and cartilage construction.

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