Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (29): 5371-5374.doi: 10.3969/j.issn.1673-8225.2011.29.013

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Cytotoxicity evaluation of nano-Ag-SiO2 urethral catheter

Zhai Jun-hong1, Guo Yun-tong1, Liu Chun1, Zhang Li1, Zhang Yan-gang1, Zhang Jin2   

  1. 1First Hospital of Shanxi Medical University, Taiyuan  030001, Shanxi Province, China
    2Shanxi Medical University, Taiyuan  030001, Shanxi Province, China
  • Received:2010-12-06 Revised:2011-02-20 Online:2011-07-16 Published:2011-07-16
  • Contact: Liu Chun, Chief physician, Master’s supervior, First Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China sxtyliuchun@126.com
  • About author:Zhai Jun-hong★, Master, First Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
  • Supported by:

    the Natural Science Foundation of Shanxi Province, No. 2006011119*

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Abstract:

BACKGROUND: Antibacterial activated nano- Ag-SiO2 urethral catheter can reduce the incidence of catheter-associated urinary tract infections obviously. Because of diversification of physical and chemical properties, security shoud be re-evaluated.
OBJECTIVE: Based on comparison of nano-Ag-SiO2 urethral catheter and ordinary urethral catheter extracts on in vitro activity of rabbit epithelial cells, to perform a preliminary evaluation of nano-Ag-SiO2 urethral catheter biological safety.
METHODS: Mechanical separation and enzymatic digestion were used to isolate and culture rabbit urothelial cells to make cell suspension after in vitro proliferation. The medium was replaced by immersion nano-Ag-SiO2 and general catheter tube medium. MTT assay was performed to quantify two extracts and detect in vitro cytotoxicity of rabbit urethra epithelial cells. Absorbance values of nano-Ag-SiO2 and general urinary catheter groups were measured by enzyme-linked immunosorbent assay to calculate the relative growth rate, and compare and perform toxicity evaluation.
RESULTS AND CONCLUSION: Nano-Ag-SiO2 and general anti-infective urinary catheter were slightly toxic. The relative growth rate had no difference between the two groups (P > 0.05). Nano-Ag-SiO2 urethral catheter effect on cell growth and reproduction is same as that of general urinary catheter, and there is no or low cytotoxicity that is consistent with the biological evaluation standards of medical devices.

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