Abstract:

BACKGROUND: This study was designed to establish gene engineered cells which auto-secreting human tumor necrosis factor-α (TNF-α). Alginate-polylysine-alginate (APA) microencapsulated cells were co-cultured with colon cancer cells. On the one hand, the effects of micro-biological pump could be exerted to continously secrete TNF-α in the intracapsule cells. On the other hand, the immune rejection-prevented effects of APA microcapsules could be exerted to limit the overgrowth of highly-proliferative intracapsule cells.
OBJECTIVE: To explore the effects of co-culture of TNFα-sercreting microcysts and human colon cancer cells Lovo on the proliferation of cancer cells.
METHODS: Former established method was used. APA microcapsules were used to wrap TNF-α/293 cells and APA microencapsulated 0/293 cells. Logarithmic growth phase colon (Lovo) cells with DMEM containing 10% fetal bovine serum were prepared into cell suspension, and inoculated on 24-well plate. After 24 hours, the supernatant was discarded, and each well was added stable transfected APA microencapsulated TNF-α/293, which were divided into 5 groups including TNF-α/293 APA microencapsulated cell low dose group, middle dose group and high dose group, negative group and APA microencapsulated 0/293 cells. The positive group was added TNF-α factor, and MTT assay under the optical density 490 nm was applied. Through human tumor cell proliferation inhibition experiment, the inhibitory effects on colon cancer cells (Lovo) proliferation were observed.
RESULTS AND CONCLUSION: APA microencapsulated 0/293 cell had no inhibitory effect on colon cancer cell proliferation in vitro, and there was no significant difference (P > 0.05). The middle, high-dose groups of APA microencapsulated TNF-α/293 cells and TNF-α-positive group showed a significant lower A at 24 h, 48 h, 72 h than the APA microencapsulated 0/293 cells group, with a significant difference (P < 0.05), suggesting that the middle, high-dose groups of APA microencapsulated TNF-α/293 cells and TNF-α-positive group had significant inhibition on the proliferation of colon cancer cells. The inhibition of APA microencapsulated TNF-α/293 cells secreted TNF-α on colon cancer cell proliferation shows a good dose-effect dependency, and expresses a considerable inhibition on the colon cancer cell proliferation as positive drug.