Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (29): 5359-5362.doi: 10.3969/j.issn.1673-8225.2011.29.010

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Vascularization of autosetting calcium phosphate cultivated with recombinant human bone morphogenetic protein 2

Wang Wei1, Mayila•Wufuer2, Abudusaimi•Aimaiti1, Aihemaitijiang•Yusufu1   

  1. 1Department of Microsurgical Repair, Orthopedics Center, 2Heart Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China
  • Received:2011-04-01 Revised:2011-05-12 Online:2011-07-16 Published:2011-07-16
  • Contact: Aihemaitijiang? Yusufu, Professor, Doctoral supervisor, Department of Microsurgical Repair, Orthopedics Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China ahmatjang@163.com
  • About author:Wang Wei★, Studying for master’s degree, Department of Microsurgical Repair, Orthopedics Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China Wangwei201107@yahoo.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30960390*; Specific Research Fund of the First Affiliated Hospital of Xinjiang Medical University, No. 2007-GXB-04*

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Abstract:

BACKGROUND: Deep fascia promotes vascularization of tissue-engineered bone, which is a mature technology, but different animal species and different implant materials can result in a great difference in vascularization.
OBJECTIVE: To compare the vascularization of autosetting calcium phosphate cement cultivated with recombinant human bone morphogenetic protein 2 (rhBMP-2) and a single autosetting calcium phosphate cement implant in beagle dogs with deep fasica flaps.
METHODS: Twelve healthy adult beagle dogs were respectively implanted with autosetting calcium phosphate cement cultivated with rhBMP-2 in the left thigh (experimental sides) and a single autosetting calcium phosphate cement in right thigh (control sides). The vascularization in each condition was assessed by experiment study (Physical, Massion stain, the hematoxylin-eosin stain, ⅧRAg marked) at time intervals of 2, 4, 8 and 16 weeks after operation.
RESULTS AND CONCLUSION: In experimental groups and in control groups, vascularization was found. New vessels invaded in scaffold with time. In experimental groups, the amount of vessels and the expression of ⅧRAg were stronger than those in control groups at 2, 4, 8, and 16 weeks. The deep fasica flaps have great effect on the vascularization. The deep fasica flap binding with rhBMP-2 is proved to be the better in vascularization of autosetting calcium phosphate cement.

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