Abstract:

BACKGROUND: It has showed that deproteinized cancellous bone maintains natural network pore system and provides better cancellous compatibility than other mechanical materials except immunology.
OBJECTIVE: To fabricate xenogeneic-deproteinized-cancellous-bone scaffold and to explore the intensity of immune reaction sparked by xenogeneic deproteinized cancellous bone implanted at goats.
METHODS: We made deproteinized bone derived from pig femoral cancellous bone by physical and chemical treatments. Their morphological features, constitute components and mechanical properties were examined by scanning electron microscopy, X-rays diffraction analysis, mechanical experimental instrument and so on. Eight male goals were divided into experimental and control group according to transplant materials. Materials included xenogeneic deproteinized cancellous bone and fresh xenogeneic cancellous bone.
RESULTS AND CONCLUSION: It showed that deproteinized cancellous bone maintained natural network pore system. The main organic material was collagenⅠ and inorganic was hydroxyapatite. It hardly has any antigen and it has good histocompatibility. The mechanical strength was no significant difference between deproteinized cancellous bone and fresh cancellous bone. In experimental group, T lymphocyte subsets CD3⁺, CD4⁺, CD8⁺and CD28⁺ in blood samples had no significant difference at different time point, and there was no positive stained reddish zones in the slides of xenogeneic deproteinized cancellous bone implanted for 4 weeks at intertransverse. Xenogeneic deproteinized cancellous bone may be safe in immunology.