Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (27): 4951-4954.doi: 10.3969/j.issn.1673-8225.2011.27.003

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Differentiation of rabbit mesenchymal stem cells into chondrocytes in vitro

Yu Meng, Yu Fang, Fu Sheng-liang   

  1. Department of Orthopedics, Beijing Shijitan Hospital Affiliated to Capital Medical University, Beijing  100038, China
  • Received:2011-01-27 Revised:2011-05-19 Online:2011-07-02 Published:2011-07-02
  • About author:Yu Meng☆, Doctor, Attending physician, Department of Orthopedics, Beijing Shijitan Hospital Affiliated to Capital Medical University, Beijing 100038, China yu_meng71@yahoo.com.cn

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Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) have a multi-differentiated potential to differentiate into bone, cartilage, tendon, adipose and so on.
OBJECTIVE: To build a method of differentiation of rabbit BMSCs into chondrocytes in vitro and to determine the factors involved in the differentiation process.
METHODS: BMSCs were isolated from young rabbit femur marrow, and then planted in plastic culture flask. After stimulated by basic fibroblast growth factor (bFGF), passage 3 cells were chosen to induce into cartilage differentiation in different concentrations of transforming growth factor-β1 (TGF-β1). Tow weeks later, the morphology of chondrocytes was detected by toludine blue, type Ⅱ collagen and aggrecan assay with RT-PCR.
RESULTS AND CONCLUSION: bFGF could stimulate the proliferation of BMSCs. Induced BMSCs had typical appearance of chondrocytes, the expression of type Ⅱ collagen and aggrecan were positive. 10 μg/L TGF-β1 was the optimal concentration for cartilage differentiation.

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