Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (27): 4946-4950.doi: 10.3969/j.issn.1673-8225.2011.27.002

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A large mass of mesenchymal stem cells isolated from tooth germs

Yang Shao-guang, Xing Wen, Tian Kun, Liu Meng, Lu Shi-hong, Zhao Qin-jun, Ren Hong-ying, Chi Ying, Ma Feng-xia, Han Zhong-chao   

  1. State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin  300020, China
  • Received:2011-02-09 Revised:2011-04-09 Online:2011-07-02 Published:2011-07-02
  • Contact: Han Zhong-chao, State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China tihzchan@public.tpt.tj.cn
  • About author:Yang Shao-guang, Associate chief technician, State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China yangsg666@163.com Xing Wen★, Master, Assistant researcher, State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China xw2318@yahoo.com.cn Yang Shao-guang and Xing Wen contributed equally to this paper.
  • Supported by:

    the National 973 Program of China, No. 2011CB964800*

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Abstract:

BACKGROUND: Mesenchymal stem cells have been isolated from dental tissues such as the dental pulp, the dental follicle and the periodontal ligament. It is unclear whether mesenchymal stem cells can be isolated directly from tooth germs, the tooth precursors. 
OBJECTIVE: To isolate mesenchymal stem cells from tooth germs of aborted fetuses and to detect their biological characteristics.
METHODS: The tooth germs were dug out entirely from fetal alveolar ridge aseptically. They were cut into small pieces and digested with collagenase Ⅱ and trypsin successively. After filtering the pieces out, the cells were washed and cultured. The expanded adherent cells were studied. Cell morphology, immunophenotype and multi-potential differentiation were analyzed.
RESULTS AND CONCLUSION: After 3 passages, more than 107 homogenous, spindle-shaped adherent cells were obtained from one fetal tooth germ. These cells expressed the markers of CD105, CD73, CD90 and CD44, but they did not express CD34 and CD45. They could differentiate into osteoblasts and adipocytes in vitro, and chondrocytes in vivo. Mesenchymal stem cells can be isolated from fetal tooth germs by plastic adherence.

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