Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (24): 4437-4439.doi: 10.3969/j.issn.1673-8225.2011.24.016

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Effect of breviscapine on transforming growth factor beta 1 in human peritoneal mesothelial cells induced by peritoneal dialysis solution

Du Fei1, Zhang Ke-fei1, Gao Yuan2, Zhang Wen-hui1, Yuan Li-ying1   

  1. 1Department of Nephrology, the Third Affiliated Hospital of Zunyi Medical College, Zunyi  563000, Guizhou Province, China
    2Department of Physiology, Zhuhai Campus of Zunyi Medical College, Zhuhai  519041, Guangdong Province, China
  • Received:2010-11-01 Revised:2011-01-31 Online:2011-06-11 Published:2011-06-11
  • About author:Du Fei★, Master, Attending physician, Department of Nephrology, the Third Affiliated Hospital of Zunyi Medical College, Zunyi 563000, Guizhou Province, China feifei632@yahoo. com.cn
  • Supported by:

    Foundation for Excellent Young Talents in Medicine from Guizhou Provincial Health Committee, No. gzwkj2009-2-009*

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Abstract:

BACKGROUND: Based on the understanding of breviscapine effects and mechanism on inhibiting and delaying peritoneal failure, breviscapine is expected to be widely introduced to prolong peritoneal dialysis time, improve the quality of dialysis, and reduce dialysis failure rate so as to promote long-term effect of peritoneal dialysis in patients with end stage renal diseases.
OBJECTIVE: To observe the effects of breviscapine on the secretion of transforming growth factor beta 1 (TGF-β1) and proliferation activity of human peritoneal mesothelial cells (HPMCs) cultured in vitro with peritoneal dialysis solution (PDS).
METHODS: HPMCs were cultured in vitro and divided into five groups: control group, PDS group, 5 µmol/L breviscapine group, 10 µmol/L breviscapine group, and 20 µmol/L breviscapine group. The levels of TGF-β1 and proliferation of HPMCs in supernatant were detected and compared.
RESULTS AND CONCLUSION: Significantly increased levels of TGF-β1 and decreased cell proliferation activity were observed in the PDS group. The level of TGF-β1 in the PDS group was higher than that in the 5 µmol/L breviscapine group (P < 0.05), and significantly higher than that in the 10 µmol/L breviscapine group and 20 µmol/L breviscapine group (P < 0.01). The proliferation activity in the PDS group was lower than that in the 5 µmol/L breviscapine group (P < 0.05), and significantly lower than that in the 10 µmol/L breviscapine group and 20 µmol/L breviscapine group (P < 0.01). The results indicated that breviscapine can reduce the secretion of TGF-β1 in cultured HPMCs. Also it can restore the reduced cell proliferation activity caused by PDS．

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