Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (24): 4383-4386.doi: 10.3969/j.issn.1673-8225.2011.24.004

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Expression of bone morphogenetic protein-2 during the distraction process in midpalatal suture of rats

Hu Hai-kun1, Zhou Jing2, Wang Yao1, Li Jing1, He Wu-lin1, Zou Shu-juan1   

  1. 1State Key Laboratory of Oral Diseases, Department of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu  610041, Sichuan Province, China
    2Department of Stomatology, Yan’an Hospital, Kunming  650031, Yunnan Province, China
  • Received:2010-12-19 Revised:2011-02-23 Online:2011-06-11 Published:2011-06-11
  • Contact: Zou Shu-juan, Doctor, Professor, Doctoral supervisor, State Key Laboratory of Oral Diseases, Department of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China shujuanzou@yahoo. com.cn
  • About author:Hu Hai-kun☆, Studying for doctorate, State Key Laboratory of Oral Diseases, Department of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China helen-8591@163. com
  • Supported by:

     the National Natural Science Foundation of China, No. 30772454*; the Tackle Key Program of Sichuan Provincial Science and Technology Bureau, No. 2006z09-013*

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Abstract:

BACKGROUND: Orthodontists usually use palatal suture expansion to solve the problem of maxillofacial deformity. Some studies have shown that bone morphogenetic protein-2 (BMP-2) can induce bone and cartilage formation, promote the distraction osteogenesis in the process of bone remodeling under mechanical tension forces. However, underlying mechanisms of BMP-2 that drive bone formation during palatal suture expansion remain unknown.
OBJECTIVE: To investigate the law of BMP-2 expression in mid-palatal suture remodeling after subjecting to tensional strain loading.
METHODS: Wistar male rats of 5 weeks old, with average weight of 70-80 g, were randomly divided into 2 groups, separately, Rats in experimental group were placed on the expansion appliance, with opening loops with an initial force of 50 g applied to mid-palatal suture for periods of 1, 4 ,7 and 14 days. After expansion, the mid-palatal suture was observed by IHC staining and real time-PCR to investigate the expression of BMP-2 protein and mRNA during mid-palatal suture remodeling after suture expansion.
RESULTS AND CONCLUSION: Sections with IHC staining revealed BMP-2 was detected in the osteoblast, osteocytes, chondrocytes, also in the connective tissue cells of suture. Coincident with IHC, the result of real-time PCR demonstrated time-differential expression of the mRNA that was significantly higher than that of controls at all-time points during the expansion suture remodeling. It is suggesting that BMP-2 was susceptible to mechanical tension forces and may play an important role in the remodeling of mid-palate suture.

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