Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (24): 4375-4378.doi: 10.3969/j.issn.1673-8225.2011.24.002

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Effect of the small molecular weight peptide on expression of osteoprotegerin and receptor activator of nuclear factor-kappa B ligand in MC3T3-E1 cells

Wu Kun-peng1, Yang Qiao-zhu1, Ma Feng-yan1, Dai Yong-wu1, Liu Yi2   

  1. 1The First Clinical Institute, Guangdong Medical College, Zhanjiang  524023, Guangdong Province, China
    2Guangdong Key Laboratory for Research and Development of Nature Drugs, Zhanjiang  524023, Guangdong Province, China
  • Received:2011-01-21 Revised:2011-01-29 Online:2011-06-11 Published:2011-06-11
  • Contact: Liu Yi, Master, Associate researcher, Guangdong Key Laboratory for Research and Development of Nature Drugs, Zhanjiang 524023, Guangdong Province, China plliu78@sina.com
  • About author:Wu Kun-peng, the First Clinical Institute, Guangdong Medical College, Zhanjiang 524023, Guangdong Province, China 67104234@163.com
  • Supported by:

    the Guangdong Provincial Scientific and Technological Plan, No. 2010B090400463*, 2009B030801339*, 2010B090400463*; the Innovative Experiment Item of Education Department of Guangdong Province, No. KY1007* ,the College Students’ Extracurricular Research of Guangdong Medical College, No.KY0701*

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Abstract:

BACKGROUND: The in vivo experiment indicated that the small molecular weight peptide (KP) can treat osteoporosis in ovariectomized rat by increasing the level of calcium (Ca) and bone mineral density. The in vitro experiment indicated that KP can induce the proliferation, differentiation and mineralization of MC3T3-E1 cells by inhibiting nuclear factor-κ B p50 and p65. It is unknown whether KP affects the expression of osteoprotegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL) in osteoblasts.
OBJECTIVE: To investigate the effect of KP on the expression of OPG and RANKL in MC3T3-E1.
METHODS: MC3T3-E1 were cultured with various concentrations of KP (0, 50 and 100 mg/L). Osteoblasts cultured without KP were assigned as control. Collected cells and analyzed the expression of OPG and RANKL with Western Blot after treating for 3, 6, 12, 18, 24, and 30 days.
RESULTS AND CONCLUSION: After 50 mg/L and 100 mg/L KP treatment, the OPG expressions in MC3T3-E1 cells were gradually increased (P < 0.01), and KP had no effect on the expression of RANKL. Compared with control, KP obviously increased the OPG/RANKL ratio (P < 0.01). Therefore, we think that KP affects the system of OPG/RANKL and indirectly inhibits osteoclast number and functions by increasing the expression of OPG, but RANKL.

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