Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (23): 4344-4350.doi: 10.3969/j.issn.1673-8225.2011.23.038

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Progress regarding induction of pluripotent stem cells

Wang Ge1, Chen Zhe1, Wu Dong-cheng2   

  1. 1Wuhan University, 2Department of Biochemistry, Wuhan University, Wuhan Hongqiao Brain Hospital, Wuhan  430071, Hubei Province, China
  • Received:2010-12-02 Revised:2011-02-20 Online:2011-06-04 Published:2011-06-04
  • Contact: Wu Dong-cheng, Doctoral supervisor, Department of Biochemistry, Wuhan Hongqiao Brain Hospital, Wuhan University, Wuhan 430071, Hubei Province, China bcdcwu@hotmail.com
  • About author:Wang Ge, Wuhan University, Wuhan 430071, Hubei Province, China gege3192@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30670813*

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Abstract:

BACKGROUND: There are large research and clinical application limitations on embryonic stem cells (ESCs) considering its potential oncogenicity and ethical disputes. In 2006, Yamanaka found that four factors (Oct3/4, Sox2, Klf4, c-Myc) could induce mouse embryonic fibroblasts into an embryonic stem cell-like state, which is called induced pluripotent stem cells (iPSCs). This shows the appearance of a new kind of embryonic stem cell-like cells.
OBJECTIVE: To understand progress and application of iPSCs in the field of stem cells.
METHODS: The first author searched PubMed database (http://www.ncbi.nlm.nih.gov/PubMed) and Wanfang database (http://g.wanfangdata.com.cn/) for literatures concerning iPSCs generation, characteristics, and research progress from 2006 to 2010. Key words were “induced pluripotent stem cells, defined factors, reprogramming, vectors, disease”. Duplicate articles were excluded. Finally, 69 articles were selected.
RESULTS AND CONCLUSION: Great progress has been made on iPSCs, includes the kind of factors, vectors, reprogramming efficiency and application research. However, there are some obstacles on somatic cells reprogramming such as potential damage of exogenous factors and vectors to cells low reprogramming efficiency. Once these problems solved, iPSCs can be widely used for disease modeling, drug test, cell transplantation and production of patient-specific iPSCs.

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