Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (20): 3675-3678.doi: 10.3969/j.issn.1673-8225.2011.20.016

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In vitro proliferation and collagen synthesis of human renal interstitial fibroblasts

Yu Guo-hua1, Kong Ling-ling2, Qu Gui-mei1, Feng Guo-ying2, Yao Wei-dong1, Lin Chun-hua3   

  1. 1Department of Pathology, 3Department of Transplant Center, Affiliated Yantai Yuhuangding Hospital, Medical College of Qingdao University, Yantai  264001, Shandong Province, China；2Yantai Campus, Binzhou Medical University, Yantai  264003, Shandong Province, China
  • Received:2010-12-13 Revised:2011-03-15 Online:2011-05-14 Published:2011-05-14
  • Contact: Yu Guo-hua★, Master, Attending physician, Department of Pathology, Affiliated Yantai Yuhuangding Hospital, Medical College of Qingdao University, Yantai 264001, Shandong Province, China ygh0535@hotmail.com
  • About author:Yu Guo-hua★, Master, Attending physician, Department of Pathology, Affiliated Yantai Yuhuangding Hospital, Medical College of Qingdao University, Yantai 264001, Shandong Province, China ygh0535@hotmail.com
  • Supported by:

    the Science and Technology Development Plan for Traditional Chinese Medicine in Shandong Province, No. 2009222*

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Abstract:

BACKGROUND: The time points for in vitro studies about renal interstitial fibroblasts are mainly selected within 72 hours. However, there are rare reported about proliferation and collagen synthesis of renal interstitial fibroblasts cultured in vitro for a long term.
OBJECTIVE: To investigate the pattern of proliferation and collagen synthesis of human renal interstitial fibroblasts in vitro.
METHODS: human renal interstitial fibroblasts were obtained, purified and identified. The third passage cells were used in experiment. Cell proliferation was determined by MTT method and collagen synthesis was observed using Masson trichrome staining accompanied with image analysis at the 1st, 3rd, 5th, 7th and 9th days after culture in vitro.
RESULTS AND CONCLUSION: The growth curve of renal interstitial fibroblasts was shown as “S” and doubling time was from the 3rd to 5th day in vitro; with the culturing time extending, the distribution of collagen protein changed from line into lamellar and the quantity of collagen protein increased obviously from the 3rd to 5th day (P < 0.05). It is indicated that the biological activity of renal interstitial fibroblasts is the most productive from the 3rd to 5th day, which might be an important target during the course of pharmacological experiment in vitro.

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